AMACR anticorps

N° du produit ABIN7538840

L’anticorps Souris Monoclonal anti-AMACR a été validé pour IHC, IHC (p) et HIER. Il convient pour détecter AMACR dans des échantillons de Humain.

Aperçu rapide pour AMACR anticorps (ABIN7538840)

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp AMACR est non-conjugé

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: EPMU1

Détail du produit anti-AMACR anticorps

Fonction: Liquid Mouse Monoclonal Antibody Alpha-Methylacyl-CoA Racemase (AMACR, p504S)

Specificité: Human alpha-methylacyl-CoA racemase.

Attributs du produit: This product is intended for the qualitative identification by light microscopy of alpha-methylacyl-CoA racemase (AMACR) molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogène: Prokaryotic recombinant protein corresponding to 382 amino acids of the human alpha-methylacyl-CoA racemase molecule.

Isotype: IgG1

Information d'application

Indications d'application: Immunohistochemistry on paraffin sections. Suggested dilution: 1:200 for 30 minutes at 25°C. Heat induced epitope retrieval using Epitope Retrieval Solution pH 9.0. This is provided as a guide and users should determine their own optimal working dilutions. Technical note: When utilised in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognized. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 % . If these recommendations are not followed, staining intensity will be reduced. It is not recommended for use with PBS, since the use of PBS based wash buffers and possibly PBS based antibody diluents gives increased background staining and decreased staining intensity. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 84 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C

Stockage commentaire: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Date de péremption: 12 months

Détails sur AMACR

Antigène: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Autre désignation: Alpha-Methylacyl-CoA Racemase

Pathways: Monocarboxylic Acid Catabolic Process