His Tag anticorps

N° du produit ABIN7539828

Cet anticorps Souris Monoclonal détecte spécifiquement His Tag dans WB, ELISA, IP, IHC, ICC et FACS. Il présente une réactivité envers Tag.

Aperçu rapide pour His Tag anticorps (ABIN7539828)

Antigène: His Tag

Reactivité: Tag

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp His Tag est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 6A8H8

Détail du produit anti-His Tag anticorps

Fonction: His Tag Antibody

Purification: Purified antibody

Immunogène: Synthesized peptide of His Tag (AA: HHHHHHHH-KLH).

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/100 - 1/1000

Restrictions: For Research Use only

Stockage

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Détails sur His Tag

Antigène: His Tag

Classe de substances: Tag

Sujet: The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells.