HSPD1 anticorps

N° du produit ABIN7540481

N° du produit (Fournisseur): 200-301-f61

Cet anticorps Souris Monoclonal détecte spécifiquement HSPD1 dans WB, IHC et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour HSPD1 anticorps (ABIN7540481)

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp HSPD1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: LK2

Détail du produit anti-HSPD1 anticorps

N° du produit (Fournisseur): 200-301-f61

Fournisseur: Rockland

Fonction: HSP60 Antibody

Réactivité croisée (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp60 from Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Pig, Rabbit, Spinach, E.

Purification: Anti-Hsp60 Antibody purified by Protein G chromatography.

Stérilité: Sterile filtered

Immunogène: Hsp60 Antibody was produced in mice by repeated immunizations raised against recombinant human Hsp60.

Isotype: IgG1

Information d'application

Indications d'application:

Immunohistochemistry_Dilution: User Optimized

Flow_Cytometry_Dilution: User Optimized

Western_Blot_Dilution: 0.25 μg/mL

Commentaires:

Anti-HSP60 Antibody is tested for WB, Flow Cytometry, and IHC. Expect a band approximately ~60kDa protein corresponding to the molecular mass of Hsp60 on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user. 0.1 mM PMSF added as an inhibitor.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Détails sur HSPD1

Antigène: HSPD1 (Heat Shock 60kDa Protein 1 (Chaperonin) (HSPD1))

Autre désignation: HSPD1

Sujet: CPN60, GROEL, HLD4, Hsp 60, Hsp65, HSPD1, HuCHA60, SPG 13, 60 kDa heat shock protein, mitochondrial, 60 kDa chaperonin, Chaperonin 60, CPN60, Heat shock protein 60, HSP-60, Hsp60, Mitochondrial matrix protein P1, P60 lymphocyte protein, PD1,In both prokaryotic and eukaryotic cells, the misfolding and aggregation of proteins during biogenesis and under conditions of cellular stress are prevented by molecular chaperones. Members of the HSP60 family of heat shock proteins are some of the best characterized chaperones. Hsp60, also known as Cpn60 or GroEl, is an abundant protein synthesized constitutively in the cell that is induced to a higher concentration after brief cell shock. It is present in many species and exhibits a remarkable sequence homology among various counterparts in bacteria, plants, and mammals with more than half of the residues identical between bacterial and mammalian Hsp60. Whereas mammalian Hsp60 is localized within the mitochondria, plant Hsp60, or otherwise known as Rubisco-binding protein, is located in plant chloroplasts. It has been indicated that these proteins carry out a very important biological function due to the fact that Hsp60 is present in so many different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo-oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of Mg2+ and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single-ring human mitochondrial homolog, Hsp60 with its co-chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co-chaperonin, GroES. Another important function of Hsp60 and Hsp10 is their protective functions against infection and cellular stress. Hsp60 has however been linked to a number of autoimmune diseases, as well as Alzheimer's, coronary artery diseases, MS, and diabetes.

ID gène: 3329

NCBI Accession: NP_002147

UniProt: P10809

Pathways: Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity