EBI3 anticorps

N° du produit ABIN7540540

N° du produit (Fournisseur): 210-301-c56s

Cet anticorps anti-EBI3 est un anticorps Souris Monoclonal détectant EBI3 dans WB, ELISA, IP et Neut. Adapté pour Souris.

Aperçu rapide pour EBI3 anticorps (ABIN7540540)

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Reactivité: Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp EBI3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Neutralization (Neut)

Clone: V1-4F5-25

Détail du produit anti-EBI3 anticorps

N° du produit (Fournisseur): 210-301-c56s

Fournisseur: Rockland

Fonction: IL-35 EBI3 Antibody

Réactivité croisée (Details): This antibody is specific for mouse EBI3 protein.

Purification: Anti-EBI-3 was purified from concentrated tissue culture supernate by Protein A chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène: Anti-EBI-3 (MOUSE) Monoclonal Antibody was produced in mouse by repeated immunizations with mature full length recombinant mouse EBI-3 produced in E.coli followed by hybridoma development.

Isotype: IgG2b

Information d'application

Indications d'application:

Neutralization_Dilution: 10 μg/mL

Immunoprecipitation_Dilution: 1 μg/mL

ELISA_Dilution: 1:10,000

Western_Blot_Dilution: 1:1000

Commentaires:

EBI-3 antibody has been tested for use in IP and Neutralization. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur EBI3

Antigène: EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))

Autre désignation: Ebi3

Sujet: Mouse anti-EBI3 antibody, mouse anti-IL-35 antibody, Epstein-Barr virus induced 3 protein, Interleukin-27 subunit beta, IL-27 subunit beta, IL-27B, Epstein-Barr virus-induced gene 3 protein, EBV-induced gene 3 protein, EBI-3, EBI3, IL-35, IL35,The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-27 p28 or IL-12 p35 subunit. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R.

ID gène: 50498

NCBI Accession: NP_056581

UniProt: O35228