PINK1 anticorps (C-Term)

N° du produit ABIN7540589

N° du produit (Fournisseur): 600-401-gu5

Cet anticorps Lapin Polyclonal détecte spécifiquement PINK1 dans WB, IHC, ELISA, IF, IP, FACS et FM. Il présente une réactivité envers Humain et a été mentionné dans 1 publication.

Aperçu rapide pour PINK1 anticorps (C-Term) (ABIN7540589)

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PINK1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Détail du produit anti-PINK1 anticorps

Épitope: C-Term

N° du produit (Fournisseur): 600-401-gu5

Fournisseur: Rockland

Fonction: PINK1 truncated Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against human PINK1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

Immunoprecipitation_Dilution: User Optimized

ELISA_Dilution: User Optimized

Immunohistochemistry_Dilution: 1:200

Flow_Cytometry_Dilution: User Optimized

IF_Microscopy_Dilution: 20 μg/mL

Western_Blot_Dilution: 1 μg/mL

Commentaires:

Suggested Applications: FC
Anti-PINK1 truncated Antibody has been tested in Western Blot, Immunofluorescence, and ELISA. Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1μg/mL in western blot. Expect a lower strength band at 62.7 kDa in western blot using appropriate lysates. PINK1 truncated antibody strongly detects endogenously truncated PINK1 protein, seen at ~30kDa. PINK1 is highly phosphorylated and degrades rapidly which may cause additional banding in western blot. Positive control cell lines for immunofluorescence assays are NIH3T3 (p/n W10-000-358). Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Date de péremption: 12 months

Références pour anticorps anti-PINK1 (ABIN7540589)

Chen, Katsuyama, Satyam, Li, Rubio, Jung, Andrzejewski, Becherer, Tsokos, Abdi, Tsokos: "CD38 reduces mitochondrial fitness and cytotoxic T cell response against viral infection in lupus patients by suppressing mitophagy." dans: Science advances, Vol. 8, Issue 24, pp. eabo4271, (2022) (PubMed).

Détails sur PINK1

Antigène: PINK1 (PTEN Induced Putative Kinase 1 (PINK1))

Autre désignation: PINK1

Sujet: Rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase,PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.

ID gène: 65018

NCBI Accession: NP_115785

UniProt: Q9BXM7

Pathways: Autophagy