SMAD1 anticorps (Internal Region)

N° du produit ABIN7540633

N° du produit (Fournisseur): 600-401-mn6s

Cet anticorps anti-SMAD1 est un anticorps Lapin Polyclonal détectant SMAD1 dans WB, ELISA, IHC, IF et FM. Adapté pour Souris.

Aperçu rapide pour SMAD1 anticorps (Internal Region) (ABIN7540633)

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SMAD1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Détail du produit anti-SMAD1 anticorps

Épitope: Internal Region

N° du produit (Fournisseur): 600-401-mn6s

Fournisseur: Rockland

Fonction: Smad1 Antibody

Réactivité croisée (Details): This affinity purified antibody is directed against mouse Smad1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Stérilité: Sterile filtered

Immunogène: Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Information d'application

Indications d'application:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:400-1:500

IF_Microscopy_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 5 μg/mL

Commentaires:

Anti-Smad1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~52.2kDa in western blot using appropriate tissues or lysates.  Positive control used: Mouse testis and C2C12 in Western Blot, C2C12 in Immunofluorescence, Mouse Lung, Skeletal Muscle, and Testis in Immunohistochemistry.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur SMAD1

Antigène: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Autre désignation: Smad1

Sujet: Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD 1, Smad1, Madh1, Madr1,Smad1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

ID gène: 17125

NCBI Accession: NP_032565

UniProt: P70340

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development