Recombinant Fc gamma RII (CD32) anticorps

N° du produit ABIN7542954

L’anticorps Rat Chimeric anti- a été validé pour FACS, WB, IHC, IF, St et BP. Il convient pour détecter dans des échantillons de Souris.

Aperçu rapide pour Recombinant Fc gamma RII (CD32) anticorps (ABIN7542954)

Antigène: Fc gamma RII (CD32)

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Rat

Clonalité: Chimeric

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Stimulation (St), Blocking Peptide (BP)

Clone: AT130-5

Détail du produit

Fonction: Anti-CD32 [AT130-5], Rat IgG1, kappa

Specificité: This antibody is specific for murine CD32, an Fc gamma surface receptor protein with a low affinity for IgG antibodies that expresses on B cells, monocytes/macrophages, NK cells, granulocytes, mast cells, and dendritic cells. CD32 binds antibody-antigen immune complexes and mediates adaptive immune responses.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c FcgammaRIIB^-/- mice with FcgammaRIIB-rat CD4 domain 3/4 fusion protein.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The original version of this antibody (mouse IgG1) was used in various FACS, Western blot, and immunofluorescence experiments to demonstrate that, for example, interaction with FcγRIIB is critical for the agonistic activity of anti-CD40 mAb (White et al., 2011, PMID: 21742972), and that immunotherapy targeting murine FcγRIIB is limited by mAb consumption and receptor internalization (Williams et al., 2013, PMID: 24026082). It is noted that, unlike the antagonistic anti-CD32 (clone AT128) antibody, which blocks FcγRIIB receptor phosphorylation, this agonistic anti-CD32 (clone AT130-5) has been shown to activate receptor function while blocking immune complex engagement to the inhibitory FcγRs (Vollack et al., 2017, PMID: 28492697). These differential antagonistic/agonistic anti-CD32 mAbs have been used to probe more specifically the role of CD32 in the murine FVIII-specific recall response (Vollack et al., 2017, PMID: 28492697).

Commentaires:

This chimeric rat antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: FcγRIIB, FcgRIIb, CD32B, CDw32, FCG2, FCGR2, FCGR2B, IGFR2, Immunoglobulin G Fc receptor II, Low affinity immunoglobulin gamma Fc region receptor II-b, MGC23887, MGC30032, RP11-5K23.6

UniProt: P08101