Recombinant CA9 anticorps

N° du produit ABIN7542959

Cet anticorps Souris Monoclonal détecte spécifiquement CA9 dans WB, IHC, ELISA, IF, FACS, BP et Crys. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant CA9 anticorps (ABIN7542959)

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CA9 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Blocking Peptide (BP), Crystallization (Crys)

Clone: M75

Détail du produit anti-CA9 anticorps

Fonction: Anti-Carbonic Anhydrase IX [M75], Mouse IgG1, kappa

Specificité: This antibody is specific for the sequence PGEEDLP, which is repeated several times between the 61st and 96th aa residues of human carbonic anhydrase IX's PG domain.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG2b

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/C mice with MaTu-infected HeLa cells.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The original format of this antibody (mouse IgG2b) was characterized by and used in IB (WB), IF, and FC assays (Pastoreková et al., 1992, PMID: 1312272). This antibody's epitope has been determined by a competitive ELISA against the peptide APGEEDLPA. Furthermore, it was used for WB and was shown to blocks the capacity of immobilized MN/CA IX to attach cells (Závada et al., 2000, PMID: 10839295). The Fab version of this antibody was crystallized and analyzed. Additionally, an scFv version of this antibody was created, and its thermodynamic parameters against the peptide DLPGEEDLPGEE were evaluated via ITC. Its K_d was determined to be 0.42 μM (Král et al., 2008, PMID: 18041760). This antibody was used for WB in breast cell lines and for IHC in xenograft tumors grown from PC-3 cells in mice (Li et al., 2009, PMID: 19538935).

Commentaires:

This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur CA9

Antigène: CA9 (Carbonic Anhydrase IX (CA9))

Autre désignation: Carbonic Anhydrase IX

Sujet: CA9, CAIX, MN/CA IX, endogenous protein p54/58N

UniProt: Q16790