Recombinant Transferrin Receptor anticorps

N° du produit ABIN7543000

Cet anticorps anti-Transferrin Receptor Monoclonal Souris (Clone OX-26) (ABIN7543000) détecte spécifiquement Transferrin Receptor dans FACS, WB, IF, IHC (fro) et in vivo. L’anticorps est réactif avec des échantillons de Humain et Rat.

Aperçu rapide pour Recombinant Transferrin Receptor anticorps (ABIN7543000)

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Transferrin Receptor est non-conjugé

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), In vivo Studies (in vivo)

Clone: OX-26

Détail du produit anti-Transferrin Receptor anticorps

Fonction: Anti-CD71 [OX-26], Mouse IgG2a, kappa

Specificité:

This antibody is specific for the rat transferrin receptor, also known as CD71.

Application Notes original Clone: The original format of this antibody (mouse IgG2a) was developed and demonstrated to effectively labele transferrin receptor-expressing cells, including dividing lymphocytes and erythroid precursors, with minimal binding to non-dividing lymphoid cells or mature erythrocytes. It was used for fluorescence-based cell sorting to isolate subpopulations of rat hematopoietic stem and progenitor cells from bone marrow and fetal liver (Jefferies et al., 1985, PMID: 2981766). The original format of the antibody was employed in immunohistochemical staining of cryostat sections from rat brain. In vivo testing in PVG/c rats involved injecting the antibody intravenously, followed by cryostat sectioning and staining. The results showed clear labelling of rat brain capillaries, especially in the cerebellum and cortex, with weaker labelling after 16 hours. No such labelling was observed in other rat tissues, supporting the specificity for brain capillaries (Jefferies et al., 1984, PMID: 6095085). This antibody was utilized as a drug carrier to deliver compounds across the blood-brain barrier (BBB) in vivo in Sprague-Dawley rats. The antibody demonstrated selective, dose-dependent binding to brain capillary endothelial cells when administered intravenously, with localization observed as early as 5 minutes post-injection and evidence of transcytosis into the brain parenchyma over time. When conjugated with methotrexate (MTX), the antibody retained its targeting capabilities, facilitating significant accumulation of the drug in the brain parenchyma compared to free MTX (Friden et al., 1991, PMID: 2052557). This antibody was used to deliver nerve growth factor (NGF) across the BBB in in vivo experiments with aged Fischer 344 rats. The OX-26-NGF conjugate was administered intravenously and demonstrated significant therapeutic effects, including enhanced cholinergic markers in grafted tissue, improvement in spatial learning in aged rats, and upregulation of NGF receptors (Granholm et al., 1998, PMID: 9683326). Mutational screening showed that this antibody's crossing of the BBB is dependent on the its affinity to the receptor (Thom et al., 2018, PMID: 29485883). The antibody can also be used in flow cytometry, immunofluorescence, and western blot, and it may also be used in frozen-section IHC.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with phytohaemagglutinin (PHA)-activated PVG rat lymph node cells with NS 1/1.Ag 4.1 myeloma cells.

Isotype: IgG2a, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Transferrin Receptor

Antigène: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Autre désignation: CD71

Sujet: Transferrin receptor, TfR, p90, T9, TFR1, OX26, MRC OX26, MRC OX-26

UniProt: Q99376

Pathways: Transition Metal Ion Homeostasis