Recombinant Podoplanin anticorps (AA 38-51)

N° du produit ABIN7543076

Cet anticorps Chèvre Chimeric détecte spécifiquement Podoplanin dans WB, IHC, ELISA, FACS et BR. Il présente une réactivité envers Souris.

Aperçu rapide pour Recombinant Podoplanin anticorps (AA 38-51) (ABIN7543076)

Antigène: Podoplanin (PDPN)

Type d'anticorp: Recombinant Antibody

Reactivité: Souris

Hôte: Chèvre

Clonalité: Chimeric

Conjugué: Cet anticorp Podoplanin est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: PMab-1

Détail du produit anti-Podoplanin anticorps

Épitope: AA 38-51

Fonction: Anti-Podoplanin (MAP tag) [PMab-1], Goat IgG, kappa

Specificité: This antibody is specific for amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin (PDPN). This antibody, therefore, possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), which is derived from the PLAG domain of murine podoplanin. PDPN is a type I transmembrane protein that interacts with the platelet receptor C-type lectin-like receptor-2 (CLEC-2). PDPN is expressed by a wide variety of cells, including podocytes, lymphatic endothelial cells and type I alveolar cells. PDPN is also upregulated on certain cancer cells, where it mediates tumour-induced platelet aggregation by binding to CLEC-2 on platelets. Chihara (2018) associated Podoplanin with a co-inhibitory receptor in T-cell activation along with PD-1, TIM-3, LAG-3 and TIGIT.

Attributs du produit:

Original Species of Ab: Rat

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogène: This antibody was raised by immunising rats with 14-residue synthetic peptide mpp3851, which corresponds to amino acids 38-51 (GDGMVPPGIEDKIT) of the platelet-aggregation-stimulating (PLAG) domain of mouse podoplanin. Spleen cells were then harvested, and fused to P3U1 cells to generate stable hybridomas.

Isotype: IgG kappa

Information d'application

Indications d'application: This antibody binds to the PLAG domain of murine PDPN and is, therefore, able to block its interaction with C-type lectin-like receptor-2 (CLEC-2) (Yamada, 2017). This has a number of potential therapeutic applications, including the suppression of lymphangiogenesis and inflammatory macrophage infiltration at the site of wound healing in mouse corneal suture and ear section models (Maruyama et al, 2014), as well as suppression of transplant rejection. This antibody possesses high affinity and specificity for the MAP epitope tag (GDGMVPPGIEDK), and can, therefore, be used to detect and purify tagged proteins in a range of assay formats (Fujii et al, 2016). PMab-1 performs well in immunohistochemical analysis, and has been used to stain the mouse melanoma B16-F10 and mouse podoplanin-expressing CHO transfectants (Kaji et al, 2012). The use of this antibody in immunostaining of somatic tissues, including mouse lung lymphatic vessels, kidney glomeruli, pulmonary alveoli, pulmonary pleura and salivary gland myoepithelial cells, has been successfully performed on both paraffin-embedded sections and frozen sections (Kaji et al, 2012). The specificity of this antibody has been confirmed in Western blot analysis (Kaji et al, 2012), while Western blot analysis has also revealed that activation of LPS-induced inflammatory pathways, including the MAPK and NF-κB pathways, is affected by PMab-1 (Maruyama et al, 2014). The mouse-rat chimeric antibody mPMab-1 is derived from PMab-1 (Yamada et al, 2017).

Commentaires:

This chimeric goat antibody was made using the variable domain sequences of the original rat IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Podoplanin

Antigène: Podoplanin (PDPN)

Autre désignation: Podoplanin

Sujet: PDPN, Aggrus, Glycoprotein 38, Gp38, OTS-8, Ots8, PA2.26 antigen, T1-alpha, T1A, Transmembrane glycoprotein E11

UniProt: Q62011

Pathways: Dicarboxylic Acid Transport