Recombinant Fc gamma RII (CD32) (F(ab')2 Region) anticorps

N° du produit ABIN7543151

Cet anticorps Souris Monoclonal détecte spécifiquement Fc gamma RII dans FACS, ELISA et BR. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Fc gamma RII (CD32) (F(ab')2 Region) anticorps (ABIN7543151)

Antigène: Fc gamma RII (CD32)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: MDE-8

Détail du produit

Épitope: F(ab')2 Region

Fonction: Anti-CD32 [MDE-8], Mouse IgG1, kappa

Specificité:

This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes, polymorphonuclear cells and platelets.

Application Notes original Clone: The binding ability of MDE-8 to FcgammaRIIa was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines, peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this receptor. It can also effectively blocked FcγRIIa ligand-binding via its F(ab')2 fragment. Overnight incubation of monocytes with F(ab')2 fragments of MDE-8 leads to a 60 % decrease in cell surface expression of FcγRIIa. MDE-8 whole antibody induced a concomitant 30 % decrease of FcγRI on THP-1cells and monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotype: IgG1, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318