Recombinant Fc gamma RII (CD32) anticorps

N° du produit ABIN7543154

Cet anticorps Lapin Chimeric détecte spécifiquement dans FACS, IHC, ELISA, IF et BR. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant Fc gamma RII (CD32) anticorps (ABIN7543154)

Antigène: Fc gamma RII (CD32)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Inconjugué

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Blocking Reagent (BR)

Clone: MDE-9

Détail du produit

Fonction: Anti-CD32 [MDE-9], Rabbit IgG, kappa

Specificité: This antibody recognizes and binds Fc-gamma receptor IIa (FcγRIIa) or CD32a and, more particularly, the FcγRIIa-H131 allotype.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum albumin in complete Freund's adjuvant.

Isotype: IgG kappa

Information d'application

Indications d'application: MDE-9 was able to detect FcgammaRIIa-H131 in both flow cytometry and immunohistochemistry. MDE-9 bound FcgRIIa-H131 on cell lines expressing FcgRIIaH131, as well as on peripheral blood monocytic cells and PMN in immunofluorescence assays. MDE-9 was used to develop an FcgammaRIIa allotyping method based on flow cytometry. The results of flow cytometric FcgammaRIIa allotyping correlated completely with PCR genotyping. This novel allotyping assay can facilitate the screening of patients in a routine diagnostic setting. A combination of MDE-9 and mAb 41H16 can be used in FcgammaRIIa-H/H131 homozygous individuals to detect FcgammaRIIa and FcgammaRIIb surface expression on monocytes (PMID: 15604023).

Commentaires:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détail du antigène

Antigène: Fc gamma RII (CD32)

Autre désignation: CD32

Sujet: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, FcgammaRIIa-H131, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318