Recombinant TREM1 anticorps

N° du produit ABIN7543260

L’anticorps anti-TREM1 Chimeric Lapin est utilisé pour la détection de TREM1 dans des échantillons de Humain, Cynomolgus et Macaque rhésus. Il a été validé pour FACS, ELISA et BR.

Aperçu rapide pour Recombinant TREM1 anticorps (ABIN7543260)

Antigène: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain, Cynomolgus, Macaque rhésus

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp TREM1 est non-conjugé

Application: Flow Cytometry (FACS), ELISA, Blocking Reagent (BR)

Clone: 14F69

Détail du produit anti-TREM1 anticorps

Fonction: Anti-TREM-1 [14F69], Rabbit IgG, kappa

Specificité:

This antibody binds human, cynomolgus monkey and rhesus monkey TREM-1. TREM1 is a receptor that is expressed on monocytes, macrophages, neutrophils and dendritic cells. It plays important roles in innate and adaptive immunity by amplifying inflammatory responses. When activated, TREM-1 associates with the ITAM-containing signaling adaptor protein, DAP12. Downstream signalling may include activation of the NFAT transcription factor, causing an upregulation of pro-inflammatory cytokine production.

Application Notes original Clone: The original IgG2a antibody binds human TREM-1 with an affinity of 2 X 10^-10 nM as tested by suface plasmon resonance. The screening of original antibody for binding human TREM-1 was done using ELISA and flow cytometry. This antibody is capable of binding both human, cynomolgus monkey and rhesus monkey PBMCs. This antibody was reported to block the release of TNFalpha from stimulated peripheral blood mononuclear cells (PBMCs). This antibody was also able to block the ligand-induced TREM-1 activation in such BWZ/hTREM-1 reporter cell assay (US9000127).

Réactivité croisée: Cynomolgus, Macaque rhésus

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing both wild type BALB/c mice and TREM-1 knock-out (KO) mice (C57BL/6 background) with either human TREM-1, cells expressing hTREM-1 (BWZ.36 cells), or a combination of both.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2a

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur TREM1

Antigène: TREM1 (Triggering Receptor Expressed On Myeloid Cells 1 (TREM1))

Autre désignation: TREM-1

Sujet: TREM1, CD354, Triggering receptor expressed on myeloid cells 1, Triggering receptor expressed on monocytes 1

UniProt: Q9NP99