Recombinant XCR1 anticorps

N° du produit ABIN7543269

L’anticorps anti-XCR1 Chimeric Lapin est utilisé pour la détection de XCR1 dans des échantillons de Souris et Rat. Il a été validé pour IHC, FACS et in vivo.

Aperçu rapide pour Recombinant XCR1 anticorps (ABIN7543269)

Antigène: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Type d'anticorp: Recombinant Antibody

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp XCR1 est non-conjugé

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS), In vivo Studies (in vivo)

Clone: MARX10

Détail du produit anti-XCR1 anticorps

Fonction: Anti-XCR1 [MARX10], Rabbit IgG, kappa

Specificité:

This antibody binds mouse XCR1, which is a receptor for chemokines SCYC1 and SCYC2 and expressed on 80 % of CD8+ the dendritic cells. It transduces a signal by increasing the intracellular calcium ions level. This antibody is also reported to cross-reacts with rat CXR1.

Application Notes original Clone: This antibody was used to stain cryostat sections (12 μm) of spleens from C57BL/6 WT and B6.XCR1-lacZ mice. It was found that this antibody could stain 20 % of splenic conventional dendritic cells in C57BL/6 WT mice but did not give a signal in homozygous B6.XCR1-lacZ animals, which lack the XCR1 receptor. This antibody was also used to subsets of dendritic cells that express XCR1 using flow cytometry (Bachem et al., 2012, PMID: 22826713). Digoxigenin (Dig) coupled MARX10 was injected into C57BL/6 mice for in vivo labeling of XCR1⁺ dendritic cells. Recombinant ovalbumin (OVA) coupled MARX10 (MARX10-OVA) was used to measure antibody levels in C57BL/6 and homozygous B6.XCR1-lacZ mice serum following injection and for an in vivo cytotoxicity assay, MARX10-OVA was found to be highly effective in inducing CD8⁺ T cell cytotoxicity in vivo (Hartung et al., 2015, PMID: 25520399). This antibody has been added to the Antibody Registry (RRID: AB_3668949).

Réactivité croisée: Rat

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing B6.XCR1-lacZ mice every 3 weeks (4 times in succession) with 30 x 10^6 CD11c-enriched cells from C57BL/6 WT mice in combination with heat-inactivated B. pertussis.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur XCR1

Antigène: XCR1 (Chemokine (C Motif) Receptor 1 (XCR1))

Autre désignation: XCR1

Sujet: MXCR1, Ccxcr1, Chemokine XC receptor 1, Lymphotactin receptor, SCM1 receptor, XC chemokine receptor 1, MARX1O

UniProt: Q9R0M1