Recombinant TIGIT anticorps

N° du produit ABIN7543289

Cet anticorps Souris Monoclonal détecte spécifiquement TIGIT dans ELISA, FACS et BR. Il présente une réactivité envers Humain.

Aperçu rapide pour Recombinant TIGIT anticorps (ABIN7543289)

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TIGIT est non-conjugé

Application: ELISA, Flow Cytometry (FACS), Blocking Reagent (BR)

Clone: 4A5

Détail du produit anti-TIGIT anticorps

Fonction: Anti-TIGIT [4A5], Mouse IgG1, kappa

Specificité: This antibody binds the extracellular domain of the human TIGIT (T cell immunoreceptor with Ig and ITIM domains). TIGIT is a type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins. It is an immune receptor present on some T cells and natural killer cells (NK). It binds the poliovirus receptor (PVR) with high affinity which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing 9 week old BALB/c mice with recombinant human TIGIT extracellular region Fc fusion protein in complete Freund's adjuvant.

Isotype: IgG1 kappa

Information d'application

Indications d'application: The binding characterization of this antibody towards human TIGIT was done using ELISA and flow cytometry. This antibody can bind human TIGIT extracellular domain Fc fusion protein in an ELISA and is also capable of recognizing TIGIT expressed on HEK293 cells by flow cytometry. The original IgG1 version of this antibody binds human TIGIT with a binding affinity of Kd=3.732 X 10^(-10) M. This antibody was also reported to successfully block the binding of TIGIT to its ligand CD155. This antibody is capable of binding NK cells in vitro. The anti-cancer activity of this antibody was tested on ovarian cancer cell HO8910, human non-small cell lung cancer cell NCl-H358 and human non-small cell lung cancer cell NCl-H1650 (CN109384846).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur TIGIT

Antigène: TIGIT (T Cell Immunoreceptor with Ig and ITIM Domains (TIGIT))

Autre désignation: TIGIT

Sujet: VSTM3, VSIG9, WUCAM, T-cell immunoreceptor with Ig and ITIM domains, V-set and immunoglobulin domain-containing protein 9, V-set and transmembrane domain-containing protein 3

UniProt: Q495A1

Pathways: Cancer Immune Checkpoints