Recombinant MSH2 anticorps (AA 919-934)

N° du produit ABIN7543327

Cet anticorps anti-MSH2 Chimeric Lapin (Clone 1B11-14-16) (ABIN7543327) détecte spécifiquement MSH2 dans WB, IHC et ELISA. L’anticorps est réactif avec des échantillons de Humain.

Aperçu rapide pour Recombinant MSH2 anticorps (AA 919-934) (ABIN7543327)

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp MSH2 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 1B11-14-16

Détail du produit anti-MSH2 anticorps

Épitope: AA 919-934

Fonction: Anti-MSH2 [1B11-14-16], Rabbit IgG, kappa

Specificité:

This antibody binds an epitope on the human DNA mismatch protein MSH2 between amino acids 919-934 (NSFVNEIISRIKVTT). MSH2 forms a heterodimer with MSH6 to make the human MutSα mismatch repair complex. The DNA mismatch repair system (MMR) is composed of enzyme molecules that specifically repair DNA base mismatches. MSH2 is involved in many different forms of DNA repair, including transcription-coupled repair, homologous recombination, and base excision repair. Studies have shown that mutations and/or loss of function of MMR genes are related to the occurrence of a variety of tumors, such as colorectal cancer, skin cancer, ovarian cancer, and endometrial cancer.

Application Notes original Clone: The binding specificity of this antibody for human MSH2 was determined using ELISA. This antibody was capable of detecting MSH2 from HCT8 and Biu-87 cell lysates in a western blot. This antibody was also used in the immunohistochemical analysis of normal tissues like tonsil, appendix tissues and cancerous tissues like colorectal and bladder cancer. This antibody shows similar staining of MSH2 protein compared to the commercially available antibody G219-1129 (CN110218251).

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format for improved compatibility with existing reagents assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing BALB/c mice with human MSH2 protein peptide (KLH-NSFVNEIISRIKVTT-Cys) conjugated with KLH in equal volume of Freund's complete adjuvant.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur MSH2

Antigène: MSH2 (Mismatch Repair Protein 2 (MSH2))

Autre désignation: MSH2

Sujet: HMSH2, DNA mismatch repair protein Msh2, MutS protein homolog 2, CGMCC NO.17405, G219-1129

UniProt: P43246

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response