Recombinant Lipoprotein Lipase anticorps

N° du produit ABIN7543369

Cet anticorps anti-Lipoprotein Lipase est un anticorps Souris Monoclonal détectant Lipoprotein Lipase dans WB, ELISA, ICC, IF, Neut, SPR et Crys. Adapté pour Humain.

Aperçu rapide pour Recombinant Lipoprotein Lipase anticorps (ABIN7543369)

Antigène: Lipoprotein Lipase (LPL)

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Lipoprotein Lipase est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Neutralization (Neut), Surface Plasmon Resonance (SPR), Crystallization (Crys)

Clone: 5D2

Détail du produit anti-Lipoprotein Lipase anticorps

Fonction: Anti-Lipoprotein lipase [5D2], Mouse IgG1, kappa

Specificité: The antibody is specific for lipoprotein lipase (LPL) and it binds to a tryptophan (Trp)-rich loop in the carboxyl terminus of LPL. The antibody does not cross react with mouse LPL. LPL is a key enzyme in triglyceride metabolism, it catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage.

Réactivité croisée: Cat, Poulet, Boeuf (Vache), Cobaye, Rat

Attributs du produit:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing mice with bovine LPL.

Isotype: IgG1 kappa

Information d'application

Indications d'application: LPL was immunoprecipitated from postheparin plasma using this antibody. The antibody was used both to capture LPL and to detect the bound LPL in a sandwich ELISA, indicating the epitope to be present in duplicate. In another sandwich ELISA analysis, the antibody was used to detect the LPL antigen (Peterson et al., 1992, PMID: 1279089). The antibody detected LPL from human, chicken and guinea pig by western blot analysis. The specificity of the original format of the antibody was confirmed by ELISA analysis (Chang et al., 1998, PMID: 9831623). Pretreatment of LPL with the antibody totally suppressed LPL-induced monocyte adhesion to endothelial cells (Mamputu et al., 1997, PMID: 9323582). Further, LDL was immunoneutralized with the antibody, totally suppressing LPL-induced VSMC proliferation (Mamputu et al., 2000, PMID: 11031206). Immunofluorescence was performed on LPL expressed on CHO-K1 cells using this antibody (Voss et al., 2011, PMID: 21518912). The binding affinity of the antibody to a synthetic LPL peptide containing the Trp-rich loop of human, mouse, bovine, rat and chicken, was measured by surface plasmon resonance.The binding affinity of the antibody to the human, bovine and chicken LPL peptides was high (KD of 0.19, 0.78 nM and 0.34 nM, respectively), the binding affinity for the rat LPL peptide was reduced (Kd= 2.99nM), and no binding to the Trp-rich peptide from mouse LPL was detected. The antibody detected LPL by western blot analysis. By immunocytochemistry, the antibody bound avidly to the wildtype human LPL on CHO cells. The crystal structure of the Fab version of the antibody was solved (Luz et al., 2020, PMID: 32690595).

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Lipoprotein Lipase

Antigène: Lipoprotein Lipase (LPL)

Autre désignation: Lipoprotein lipase

Sujet: LPL, Phospholipase A1, EC 3.1.1.34

UniProt: P06858

Pathways: Lipid Metabolism