Recombinant K-RAS anticorps (G12D)

N° du produit ABIN7543443

L’anticorps anti-K-RAS Chimeric Lapin est utilisé pour la détection de K-RAS dans des échantillons de Humain. Il a été validé pour ELISA.

Aperçu rapide pour Recombinant K-RAS anticorps (G12D) (ABIN7543443)

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp K-RAS est non-conjugé

Application: ELISA

Clone: D113

Détail du produit anti-K-RAS anticorps

Épitope: G12D

Fonction: Anti-KRAS G12D mutant [D113], Rabbit IgG, kappa

Specificité:

This antibody binds the G12D mutant form of human KRAS protein. This ras protein binds GDP/GTP and possess intrinsic GTPase activity, that results in conversion of nucleotide guanosine triphosphate (GTP) into guanosine diphosphate (GDP). This protein plays an important role in many signal transduction pathways and regulation of cell proliferation. Somatic KRAS mutations are highly observed in leukemias, colorectal cancer, pancreatic cancer and lung cancer. KRAS mutation is G12D which is estimated to be present in up to 37 % pancreatic cancers and over 12 % of colorectal cancers.

Application Notes original Clone: The binding reactivity and epitope characterization of this antibody towards human KRAS G12D mutant was determined using ELISA (PMID: 9440050). This antibody can bind both the peptide 'KLVVVGADGVGK' conjugated BSA as well as KRAS G12D mutant in an ELISA.

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: The original antibody was generated by immunizing mice with a synthetic dodecapeptide 'KLVVVGADGVGK'.

Isotype: IgG, kappa

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur K-RAS

Antigène: K-RAS (KRAS) (GTPase Kras (KRAS))

Autre désignation: KRAS

Sujet: K-Ras, GTPase Kras, KRAS2, RASK2, K-Ras 2, Ki-Ras, c-K-ras, c-Ki-ras, ras p21 protein, p21 ras, D113-2-1

UniProt: P01116-2