Recombinant Iba1 anticorps

N° du produit ABIN7543454

Cet anticorps Lapin Chimeric détecte spécifiquement Iba1 dans WB, ELISA, IF et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour Recombinant Iba1 anticorps (ABIN7543454)

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Type d'anticorp: Recombinant Antibody

Reactivité: Humain

Hôte: Lapin

Clonalité: Chimeric

Conjugué: Cet anticorp Iba1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: GT1-mAb1

Détail du produit anti-Iba1 anticorps

Fonction: Anti-AIF1 [GT1-mAb1], Rabbit IgG, lambda

Specificité:

This antibody is specific to < a href="https://everestbiotech.com/product/ebp05419-immunizing-peptide/" target="_blank">EBP05419, an immunizing peptide with the sequence C-TGPPAKKAISELP. This peptide was used in the production of the < a href="https://everestbiotech.com/product/goat-anti-aif1-iba1-isoform-1-and-3-antibody/" target="_blank">polyclonal Goat Anti-AIF1 antibody from which this antibody is derived.

Application Notes original Clone: This antibody (GT1-mAb1) was one of two monoclonal antibodies discovered by analyzing a polyclonal antibody (GT-1) sample. Briefly, de novo sequencing of GT-1 resulted in the full-length sequences of two distinct monoclonal antibodies (GT1-mAb1 and GT1-mAb2) in both framework and CDR regions. Both antibodies were recombinantly expressed by Absolute Antibody in HEK293 cells and tested in various assays. GT1-mAb1 was tested against the peptide antigen in an ELISA, and it showed activity comparable to that of the original polyclonal antibody. GT1-mAb1 was found to bind the target peptide in a non-competitive manner with GT1-mAb2 while being efficiently blocked by the original polyclonal antibody, proving that both monoclonal antibodies bind distinct epitopes and that GT1-mAb1 possess binding characteristics of the input polyclonal antibody. Furthermore, GT1-mAb1 was compatible with immunofluorescence (IF), flow cytometry (FC), and Western blot (WB), with an FC signal observedly more distinct than that of GT1-mAb2.

Attributs du produit: This chimeric rabbit antibody was made using the variable domain sequences of the original Goat IgG format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogène: This antibody was obtained by sequencing a polyclonal goat IgG raised against a peptide with the sequence C-TGPPAKKAISELP.

Isotype: IgG, lambda

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

OriginalSpeciesName: Goat

OriginalFormat: IgG

Restrictions: For Research Use only

Stockage

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Détails sur Iba1

Antigène: Iba1 (IBA1) (Ionized Calcium-binding Adapter Molecule 1 (IBA1))

Autre désignation: AIF1

Sujet: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1

UniProt: P55008

Pathways: Smooth Muscle Cell Migration