Biotin anticorps (FITC)

N° du produit ABIN7565751

Cet anticorps anti-Biotin Polyclonal Lapin conjugué à FITC (ABIN7565751) détecte spécifiquement Biotin dans FACS et FM.

Aperçu rapide pour Biotin anticorps (FITC) (ABIN7565751)

Antigène: Biotin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Biotin est conjugé à/à la FITC

Application: Flow Cytometry (FACS), Fluorescence Microscopy (FM)

Détail du produit anti-Biotin anticorps (FITC)

Fonction: Biotin Antibody Fluorescein Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-Rabbit Serum, Biotin conjugated IgG and Biotin conjugated Albumin.

Attributs du produit: Synonyms: rabbit anti-Biotin Antibody FITC Conjugation, Fluorescein conjugated rabbit anti-Biotin antibody, Biotin FITC, Anti-Biotin fluorescein Antibody

Purification: Anti-Biotin Antibody Fluorescein Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Biotin conjugated Keyhole Limpet Hemocyanin (KLH)

Immunogen Type: Native Protein

Labeling Ratio: 2.7

Information d'application

Indications d'application: Application Note: Anti-Biotin Antibody Fluorescein Conjugated is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Flow Cytometry Dilution: User Optimized IF Microscopy Dilution: 1:50:1:300 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Biotin

Antigène: Biotin

Classe de substances: Chemical

Sujet: Background: Biotin Antibody detects Biotin. Biotin is a water-soluble B-complex vitamin (vitamin B7). It is composed of a ureido (tetrahydroimidizalone) ring fused with a tetrahydrothiophene ring. A valeric acid substituent is attached to one of the carbon atoms of the tetrahydrothiophene ring. Biotin is a coenzyme for carboxylase enzymes, involved in the synthesis of fatty acids, isoleucine, and valine, and in gluconeogenesis. Biotin is necessary for cell growth, the production of fatty acids, and the metabolism of fats and amino acids. Anti-Biotin Fluorescein Conjugated Antibody is ideal for investigators involved in Cell Signaling and Cell Biology research.