PAX6 anticorps

N° du produit ABIN7565804

L’anticorps Souris Monoclonal anti-PAX6 a été validé pour WB, ELISA et FM. Il convient pour détecter PAX6 dans des échantillons de Humain.

Aperçu rapide pour PAX6 anticorps (ABIN7565804)

Antigène: PAX6 (Paired Box 6 (PAX6))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PAX6 est non-conjugé

Application: Western Blotting (WB), ELISA, Fluorescence Microscopy (FM)

Clone: 7H10-D3-D4-G5

Détail du produit anti-PAX6 anticorps

Fonction: PAX6 Antibody

Réactivité croisée (Details): Cross-reactivity with PAX6 from other sources has not been determined.

Attributs du produit: Synonyms: mouse anti-pax6 antibody, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin, AN2, PAX 6 Antibody

Purification: Anti-PAX6 was purified from clarified ascites by Protein A chromatography.

Stérilité: Sterile filtered

Immunogène:

Immunogen: PAX6 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to human PAX6 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG1 kappa

Information d'application

Indications d'application: Application Note: Anti-PAX6 antibody has been tested by ELISA and Immunofluorescence and suitable for Western Blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 46.7 kDa in size corresponding to PAX6 by western blotting in the appropriate cell lysate or extract. Western Blot Dilution: 1:500 - 1:2,000 ELISA Dilution: 1:75,000 - 1:150,000 IF Microscopy Dilution: 1:100

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur PAX6

Antigène: PAX6 (Paired Box 6 (PAX6))

Autre désignation: PAX6

Sujet: Background: Paired box protein Pax-6 also known as aniridia type II protein (AN2) or oculorhombin is a transcription factor with key functions in the development of the eye, nose, central nervous system and pancreas. Pax6 further seems to be required for the differentiation of pancreatic islet alpha cells. PAX6 has been shown to bind to a promoter element in the glucagon, insulin and somatostatin promoters and regulates the specification of the ventral neuron subtypes by establishing the correct progenitor domains. Isoform 5a appears to function as a molecular switch that specifies target genes. Anti-PAX6 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

ID gène: 5080

NCBI Accession: NP_000271

UniProt: P26367

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis