Peroxidase anticorps (Biotin)

N° du produit ABIN7565805

Cet anticorps anti-Peroxidase est un anticorps Lapin Polyclonal détectant Peroxidase dans WB, IHC et DB. Adapté pour Raifort.

Aperçu rapide pour Peroxidase anticorps (Biotin) (ABIN7565805)

Antigène: Peroxidase

Reactivité: Raifort

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Peroxidase est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-Peroxidase anticorps (Biotin)

Fonction: Peroxidase (Horseradish) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish].

Attributs du produit: Synonyms: rabbit anti-Horseradish Peroxidase Antibody Biotin Conjugation, rabbit anti-HRP Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-HRP Antibody, Peroxidase Biotin Conjugated antibody, Anti-Peroxidase BAC Antibody

Purification: Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Peroxidase [Horseradish]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:250 Western Blot Dilution: 1:500 - 1:2,000

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Buffer: Restore with deionized water (or equivalent)

Reconstitution Volume: 100 μL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur Peroxidase

Antigène: Peroxidase

Sujet: Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Peroxidase antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.