Peroxidase anticorps (Biotin)

N° du produit ABIN7565806

Cet anticorps Lapin Polyclonal détecte spécifiquement Peroxidase dans WB, IHC et DB. Il présente une réactivité envers Raifort.

Aperçu rapide pour Peroxidase anticorps (Biotin) (ABIN7565806)

Antigène: Peroxidase

Reactivité: Raifort

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Peroxidase est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), Dot Blot (DB)

Détail du produit anti-Peroxidase anticorps (Biotin)

Fonction: Peroxidase (Horseradish) Antibody Biotin Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Peroxidase [Horseradish].

Attributs du produit: Synonyms: rabbit anti-Horseradish Peroxidase Antibody Biotin Conjugation, rabbit anti-HRP Antibody Biotin Conjugation, Biotin Conjugated rabbit anti-HRP Antibody, Peroxidase Biotin Conjugated antibody, Anti-Peroxidase BAC Antibody

Purification: Anti-Peroxidase (Horseradish) Antibody Biotin Conjugated is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Peroxidase [Horseradish]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Peroxidase Antibody Biotin Conjugated has been tested by dot blot and is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Immunohistochemistry Dilution: User Optimized Western Blot Dilution: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Peroxidase

Antigène: Peroxidase

Sujet: Background: Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Peroxidase antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.