Ascorbate Oxidase anticorps (HRP)

N° du produit ABIN7565856

Cet anticorps anti-Ascorbate Oxidase est un anticorps Lapin Polyclonal détectant Ascorbate Oxidase dans ELISA, WB et IP. Adapté pour Pumpkin.

Aperçu rapide pour Ascorbate Oxidase anticorps (HRP) (ABIN7565856)

Antigène: Ascorbate Oxidase (AO)

Reactivité: Pumpkin

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Ascorbate Oxidase est conjugé à/à la HRP

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Détail du produit anti-Ascorbate Oxidase anticorps (HRP)

Fonction: Ascorbate Oxidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum and purified and partially purified Ascorbate Oxidase [cucurbita].

Attributs du produit: Synonyms: rabbit anti-Ascorbate Oxidase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Ascorbate Oxidase Antibody, L-ascorbate oxidase, Ascorbase, ASO

Purification: Ascorbate Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Ascorbate Oxidase [cucurbita]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Ascorbate Oxidase Peroxidase Conjugated Antibody has been tested by ELISA and western blot. This product is assayed against 1.0 μg of Alkaline Phosphatase [Calf Intestine] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,000 to 1:11,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: 1:100 ELISA Dilution: 1:3,000 - 1:10,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur Ascorbate Oxidase

Antigène: Ascorbate Oxidase (AO)

Autre désignation: AAO

Sujet: Background: Ascorbate Oxidase belongs to the family of oxidoreductases, specifically those acting on diphenols and related substances as donor with oxygen as acceptor. The systematic name of this enzyme class is L-ascorbate:oxygen oxidoreductase.

UniProt: P24792