KCTD8 anticorps (HRP)
Aperçu rapide pour KCTD8 anticorps (HRP) (ABIN7565875)
Antigène
Voir toutes KCTD8 AnticorpsReactivité
Hôte
Clonalité
Conjugué
Application
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Fonction
- Dextranase Antibody Peroxidase Conjugated
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Réactivité croisée (Details)
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Dextranase [Penicillium species].
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Attributs du produit
- Synonyms: rabbit anti-Dextranase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Dextranase Antibody, dextranase, Alpha-1,6-glucan-6-glucanohydrolase, DEX
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Purification
- Dextranase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
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Stérilité
- Sterile filtered
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Immunogène
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Immunogen: Dextranase [Penicillium species]
Immunogen Type: Native Protein
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Indications d'application
- Application Note: Anti-Dextranase Peroxidase has been tested by ELISA and western blot and assayed against 1.0 μg of Dextranase [Penicillium species] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:500 - 1:5,000 Immunoprecipitation Dilution: User Optimized ELISA Dilution: 1:2,000 - 1:10,000 Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide! -
Agent conservateur
- Sodium azide
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Précaution d'utilisation
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Stock
- -20 °C
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Stockage commentaire
- Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
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Date de péremption
- 12 months
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- KCTD8 (Potassium Channel Tetramerisation Domain Containing 8 (KCTD8))
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Autre désignation
- DEX
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Sujet
- Background: Dextranase belongs to the glycosyl hydrolase 49 family. It's catalytic reaction is endohydrolysis of (1->6)-alpha-D-glucosidic linkages in dextran.
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UniProt
- P48845
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling
Antigène
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