Pol (C-Term) anticorps

N° du produit ABIN7565974

Cet anticorps Lapin Polyclonal détecte spécifiquement Pol dans WB et ELISA. Il présente une réactivité avec des échantillons de Souris.

Aperçu rapide pour Pol (C-Term) anticorps (ABIN7565974)

Antigène: Pol

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: Western Blotting (WB), ELISA

Détail du produit

Épitope: C-Term

Fonction: Orf2 Antibody

Réactivité croisée (Details): Anti-Orf2 is directed against mouse ORF2 near a c-terminus position.

Attributs du produit: Synonyms: Rabbit Anti-Orf2 Antibody, LINE-1 retrotransposable element ORF2 protein, Endonuclease reverse transcriptase, Line1 protein, Orf2p, Retrovirus-related Pol polyprotein LINE-1, Long interspersed element-1

Purification: This product is an affinity purified antibody produced by immunoaffinity chromatography using peptide coupled to agarose beads.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Affinity purified Anti-ORF2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide near the c-terminus of mouse ORF2 protein.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: This affinity purified antibody has been tested for use in ELISA and Western Blot. Specific conditions for reactivity should be optimized by the end user. Expect a band ~150 kDa in size corresponding to Orf2 by western blotting in the liver cell lysates or extract. Western Blot Dilution: 1.0 μg/mL ELISA Dilution: 1:50,000-1:150,000

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.07 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Pol

Classe de substances: Viral Protein

Sujet: Background: Anti-Orf2 Antibody was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). The multi-functional nuclear protein ORF2p is involved in reverse transcriptase and endonuclease processes. ORF2 elements are epigenetically silenced throughout embyronic development and it is suggested that mutations of L1 would result in detrimental de novo insertions into the genome, negatively affecting gene expression.  L1 elements are critical components in the mammalian genome, and much is still not known regarding these proteins. Studies implicate that line-1 is activated in fetal oocytes and  L1ORF2p may contribute to the eradication or selection of the oocytes.

ID gène: 2731767