Pol anticorps

N° du produit ABIN7565984

L’anticorps anti-Pol Polyclonal Poulet est utilisé pour la détection de Pol dans des échantillons de Souris. Il a été validé pour ELISA.

Aperçu rapide pour Pol anticorps (ABIN7565984)

Antigène: Pol

Reactivité: Souris

Hôte: Poulet

Clonalité: Polyclonal

Conjugué: Inconjugué

Application: ELISA

Détail du produit

Fonction: L1/ORF2 Antibody

Réactivité croisée (Details): Anti-L1/ORF2 Antibody is directed against three regions within the endonuclease domain of L1 ORF2 protein.

Attributs du produit: Synonyms: chicken anti-L1/ORF2 Antibody, chicken anti-L1-ORF2 Antibody, L1 (LINE-1), Long INterspersed Element , retrotransposon ORF2

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: This IgY fraction antibody was prepared from eggs of chickens laid after repeated immunizations with three synthetic peptides conjugated to keyhole limpet hemocyanin (KLH).  The peptides correspond to regions within the endonuclease domain of L1/ORF2 protein.

Immunogen Type: Conjugated Peptide

Information d'application

Indications d'application: Application Note: L1/ORF2 Antibody has been tested for use in ELISA.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 149.5 kDa in size corresponding to L1 protein by western blotting in the appropriate cell lysate or extract. ELISA Dilution: 1:20,000-1:100,000 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.21 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Pol

Classe de substances: Viral Protein

Sujet: Background: L1/ORF2 is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The L1 (LINE-1), or Long INterspersed Element , retrotransposon ORF2 is the most common open reading frame in the human genome, present in various forms in many thousands of copies. This large family of proteins includes magnesium dependent endonucleases and a large number of phosphatases involved in intracellular signaling. Both intact ORF1 and ORF2 are absolutely required for autonomous retro-transposition. ORF2 encodes an endonuclease, reverse transcriptase, and zinc knuckle domains. The expression of ORF2 appears to be tightly regulated except in germ line tissues, embryonic tissues, and certain cancers including teratomas, testicular cancers, and leukemias. This antibody is intended for use in studying control of L1 retrotransposons.

UniProt: P11369