XDH anticorps (HRP)

N° du produit ABIN7565995

Cet anticorps Lapin Polyclonal détecte spécifiquement XDH dans WB, IP et DB. Il présente une réactivité envers Boeuf (Vache).

Aperçu rapide pour XDH anticorps (HRP) (ABIN7565995)

Antigène: XDH (Xanthine Dehydrogenase (XDH))

Reactivité: Boeuf (Vache)

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp XDH est conjugé à/à la HRP

Application: Western Blotting (WB), Immunoprecipitation (IP), Dot Blot (DB)

Détail du produit anti-XDH anticorps (HRP)

Fonction: Xanthine Oxidase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Xanthine Oxidase [Bovine Buttermilk].

Attributs du produit: Synonyms: rabbit anti-Xanthine Oxidase Antibody Peroxidase Conjugation, HRP Conjugated rabbit anti-Xanthine Oxidase Antibody, Xanthine dehydrogenase antibody, Xanthine dehydrogenase/oxidase antibody, Xanthine oxidase antibody, Xanthine oxidoreductase antibody, xdh antibody, xdha antibody, XO antibody, xor antibody

Purification: Xanthine Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Stérilité: Sterile filtered

Immunogène:

Immunogen: Xanthine Oxidase [Bovine Buttermilk]

Immunogen Type: Native Protein

Information d'application

Indications d'application: Application Note: Anti-Xanthine Oxidase Peroxidase conjugated Antibody has been tested by dot blot and is suitable to be assayed against 1.0 μg of Xanthine Oxidase [Bovine Buttermilk] in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:35,000 to 1:175,000 of the reconstitution concentration is suggested for this product. Western Blot Dilution: 1:250 Immunoprecipitation Dilution: 1:100 Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détails sur XDH

Antigène: XDH (Xanthine Dehydrogenase (XDH))

Autre désignation: XDH

Sujet: Background: Xanthine Oxidase is a key enzyme in purine degradation. It catalyzes the oxidation of hypoxanthine to xanthine, xanthine to uric acid, and contributes to the generation of reactive oxygen species.

ID gène: 280960

UniProt: P80457

Pathways: Positive Regulation of Endopeptidase Activity