TRHR anticorps (C-Term, Intracellular)

N° du produit ABIN7581994

Cet anticorps Lapin Polyclonal détecte spécifiquement TRHR dans WB, IF et IC. Il présente une réactivité avec des échantillons de Rat.

Aperçu rapide pour TRHR anticorps (C-Term, Intracellular) (ABIN7581994)

Antigène: TRHR (Thyrotropin-Releasing Hormone Receptor (TRHR))

Reactivité: Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp TRHR est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Détail du produit anti-TRHR anticorps

Épitope: AA 336-349, C-Term, Intracellular

Fonction: A Rabbit Polyclonal Antibody to Thyrotropin-Releasing Hormone Receptor 1

Séquence: CKQKPTEKAA NYS

Homologie: Mouse - identical, human -12,13 amino acid residues identical

Attributs du produit: Anti-TRHR1 Antibody (ABIN7581994) is a highly specific antibody directed against an epitope of the rat Thyrotropin-releasing hormone receptor 1. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize TRH-R from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogène: CKQKPTEKAANYS, corresponding to amino acid residues 336-349 of rat Thyrotropin-releasing hormone receptor 1

Isotype: IgG

Information d'application

Indications d'application:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Détails sur TRHR

Antigène: TRHR (Thyrotropin-Releasing Hormone Receptor (TRHR))

Autre désignation: TRHR

Sujet:

Synonyms: Thyrotropin-releasing hormone receptor 1, TRH1 receptor, TRHR, Thyroliberin receptor

Description: Thyrotropin-releasing hormone (TRH) is a widely distributed tripeptide which acts as a hormone, a paracrine regulatory factor and a neurotransmitter/neuromodulator. It is expressed in many parts of the brain as well as in various peripheral tissues1,2.In the hypothalamus, TRH is secreted from the paraventricular nucleus (VPN) by TRH neurons prior to a post-translational processing from its precursor, pro-TRH. A key player in the regulation of the hypothalamus-pituitary-thyroid (HPT) axis, TRH is released into the hypophyseal portal circulation of the median eminence and reaches the pituitary, where it binds to TRH receptors, thereby stimulating the secretion of tyrothropin (thyroid-stimulating hormone, TSH). TSH, in turn, affects the thyroid to produce T4 which is subsequently converted into its biologically-active analog, T3 - a negative mediator of TRH. In the tanycytes, ependymal cells of glial origin located in close proximity to the VPN, T4 is converted to T3 by the enzyme deiodinase II (dio2) - a counterpart to deiodinase III (dio3) which conversely inactivates the above mentioned thyroid hormones, thus indirectly promoting the production of TRHs3,4.TRH receptor 1 (TRH-R1), the only identified TRH receptor in humans, is a G-protein coupled receptor (GPCR) abundant in the anterior pituitary, neuroendocrine system, autonomic nervous system and the visceral regions of the brain2,5. Like all members of GPCRs, the TRH receptor has seven transmembrane domains, and extracellular N-terminus and intracellular C-terminal tail. TRH receptor couples Gq upon activation which in turn leads to the activation of phospholipase C5.From a behavioral aspect, it was shown that TRH-coupled TRH-R1 increases cognitive arousal by directly exciting the sleep/wake system in the lateral hypothalamic area, and inhibits seizure activity in the hippocampus by facilitating GABA release. Conversely, its deficiency, as observed in mutant model mice, is linked to growth retardation, depression, and anxiety-like behavior1,6,7.

ID gène: 25570

UniProt: Q01717