IRF3 anticorps (AA 1-419)

N° du produit ABIN7599372

Cet anticorps anti-IRF3 est un anticorps Souris Monoclonal détectant IRF3 dans WB, IF, ICC et FACS. Adapté pour Souris et Rat.

Aperçu rapide pour IRF3 anticorps (AA 1-419) (ABIN7599372)

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IRF3 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 7B10

Détail du produit anti-IRF3 anticorps

Épitope: AA 1-419

Fonction: Anti-IRF3 Antibody Picoband® (monoclonal, 7B10)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-IRF3 Antibody Picoband® (monoclonal, 7B10) (ABIN7599372). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse IRF3 recombinant protein (Position: M1-I419).

Isotype: IgG2b

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
1. Bellingham, J., Gregory-Evans, K., Gregory-Evans, C. Y. Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker. Ann. Hum. Genet. 62: 231-234, 1998. 2. Doyle, S. E., Vaidya, S. A., O'Connell, R., Dadgostar, H., Dempsey, P. W., Wu, T.-T., Rao, G., Sun, R., Haberland, M. E., Modlin, R. L., Cheng, G.IRF3 mediates a TLR3/TLR4-specific antiviral gene program. Immunity 17: 251-263, 2002. 3. Stetson, D. B., Medzhitov, R. Recognition of cytosolic DNA activates an IRF3-dependent innate immune response. Immunity 24: 93-103, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur IRF3

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Autre désignation: Irf3

Sujet:

Synonyms: Solute carrier family 2, facilitated glucose transporter member 1, Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter, SLC2A1, GLUT1

Tissue Specificity: Detected in erythrocytes (at protein level). Expressed at variable levels in many human tissues.

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Poids moléculaire: 50-55 kDa

ID gène: 54131

UniProt: P70671

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades