PCK1 anticorps (AA 1-622)

N° du produit ABIN7599502

Cet anticorps Lapin Polyclonal détecte spécifiquement PCK1 dans WB, ELISA, IHC, IF, ICC et FACS. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour PCK1 anticorps (AA 1-622) (ABIN7599502)

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PCK1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-PCK1 anticorps

Épitope: AA 1-622

Fonction: Anti-PCK1/PEPC Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PCK1/PEPC Antibody Picoband® (ABIN7599502). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PCK1/PEPC recombinant protein (Position: M1-M622).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Cao, H., van der Veer, E., Ban, M. R., Hanley, A. J. G., Zinman, B., Harris, S. B., Young, T. K., Pickering, J. G., Hegele, R. A. Promoter polymorphism in PCK1 (phosphoenolpyruvate carboxykinase gene) associated with type 2 diabetes mellitus. J. Clin. Endocr. Metab. 89: 898-903, 2004. 2. Pilz, A. J., Willer, E., Povey, S., Abbott, C. M. The genes coding for phosphoenolpyruvate carboxykinase-1 (PCK1) and neuronal nicotinic acetylcholine receptor alpha-4 subunit (CHRNA4) map to human chromosome 20, extending the known region of homology with mouse chromosome 2. Ann. Hum. Genet. 56: 289-293, 1992. 3. Ting, C.-N., Burgess, D. L., Chamberlain, J. S., Keith, T. P., Falls, K., Meisler, M. H. Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20. Genomics 16: 698-706, 1993.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PCK1

Antigène: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Autre désignation: PCK1

Sujet:

Synonyms: Tripartite motif-containing protein 6, RING finger protein 89, RING-type E3 ubiquitin transferase TRIM6, TRIM6, RNF89

Tissue Specificity: Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas. .

Background: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Poids moléculaire: 69 kDa

ID gène: 5105

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis