Phospholipase C delta 3 anticorps (AA 10-702)

N° du produit ABIN7599611

L’anticorps Lapin Polyclonal anti-Phospholipase C delta 3 a été validé pour WB, ELISA, ICC, FACS et IF. Il convient pour détecter Phospholipase C delta 3 dans des échantillons de Humain.

Aperçu rapide pour Phospholipase C delta 3 anticorps (AA 10-702) (ABIN7599611)

Antigène: Phospholipase C delta 3 (PLCd3)

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Phospholipase C delta 3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF)

Détail du produit anti-Phospholipase C delta 3 anticorps

Épitope: AA 10-702

Fonction: Anti-PLCD3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PLCD3 Antibody Picoband® (ABIN7599611). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PLCD3 recombinant protein (Position: R10-Q702).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ghosh, S., Pawelczyk, T., Lowenstein, J. M. Phospholipase C isoforms delta-1 and delta-3 from human fibroblasts: high-yield expression in Escherichia coli, simple purification, and properties. Protein Expr. Purif. 9: 262-278, 1997. 2. Kim, H., Suh, P. G., Ryu, S. H., Park, S. H. Assignment of the human PLC-delta-3 gene (PLCD3) to human chromosome band 17q21 by fluorescence in situ hybridization. Cytogenet. Cell Genet. 87: 209-210, 1999.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur Phospholipase C delta 3

Antigène: Phospholipase C delta 3 (PLCd3)

Autre désignation: PLCD3

Sujet:

Synonyms: PR domain zinc finger protein 5, PR domain-containing protein 5, PRDM5, PFM2

Tissue Specificity: Widely expressed with highest levels in colon and ovary. Tends to be silenced in breast, colorectal, gastric and liver cancer tissues.

Background: 1-Phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-3 is an enzyme that in humans is encoded by the PLCD3 gene. This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids.

Poids moléculaire: 94 kDa

ID gène: 113026

UniProt: Q8N3E9

Pathways: Signalisation WNT