SEC31A anticorps (AA 101-806)

N° du produit ABIN7599630

Cet anticorps anti-SEC31A est un anticorps Lapin Polyclonal détectant SEC31A dans WB, ELISA, IHC, IF, FACS et ICC. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour SEC31A anticorps (AA 101-806) (ABIN7599630)

Antigène: SEC31A (SEC31 Homolog A (SEC31A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp SEC31A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-SEC31A anticorps

Épitope: AA 101-806

Fonction: Anti-SEC31A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-SEC31A Antibody Picoband® (ABIN7599630). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human SEC31A recombinant protein (Position: Y101-K806).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Halperin, D., Kadir, R., Perez, Y., Drabkin, M., Yogev, Y., Wormser, O., Berman, E. M., Eremenko, E., Rotblat, B., Shorer, Z., Gradstein, L., Shelef, I., Birk, R., Abdu, U., Flusser, H., Birk, O. S. SEC31A mutation affects ER homeostasis, causing a neurologic syndrome. J. Med. Genet. 56: 139-148, 2019. 2. Jin, L., Pahuja, K. B., Wickliffe, K. E., Gorur, A., Baumgartel, C., Schekman, R., Rape, M. Ubiquitin-dependent regulation of COPII coat size and function. Nature 482: 495-500, 2012. 3. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Hirosawa, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Oharo, O. Prediction of the coding sequences of unidentified human genes. XII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 355-364, 1998.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur SEC31A

Antigène: SEC31A (SEC31 Homolog A (SEC31A))

Autre désignation: SEC31A

Sujet:

Synonyms: C-type lectin domain family 9 member A, CLEC9A, UNQ9341/PRO34046

Tissue Specificity: In peripheral blood highly restricted on the surface of BDCA31+ dendritic cells and on a small subset of CD14+ and CD16- monocytes.

Background: Protein transport protein Sec31A is a protein that in humans is encoded by the SEC31A gene. The protein encoded by this gene shares similarity with the yeast Sec31 protein, and is a component of the outer layer of the coat protein complex II (COPII). The encoded protein is involved in vesicle budding from the endoplasmic reticulum (ER) and contains multiple WD repeats near the N-terminus and a proline-rich region in the C-terminal half. It associates with the protein encoded by the SEC13 homolog, nuclear pore and COPII coat complex component (SEC13), and is required for ER-Golgi transport. Monoubiquitylation of this protein by CUL3-KLHL12 was found to regulate the size of COPII coats to accommodate unusually shaped cargo. Alternative splicing results in multiple transcript variants encoding different isoforms.

Poids moléculaire: 133 kDa

ID gène: 22872

UniProt: O94979

Pathways: ER-Nucleus Signaling