Kdm6b anticorps (AA 1127-1643)

N° du produit ABIN7599752

L’anticorps Lapin Polyclonal anti-Kdm6b a été validé pour WB, ELISA et FACS. Il convient pour détecter Kdm6b dans des échantillons de Humain et Souris.

Aperçu rapide pour Kdm6b anticorps (AA 1127-1643) (ABIN7599752)

Antigène: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Reactivité: Humain, Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp Kdm6b est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-Kdm6b anticorps

Épitope: AA 1127-1643

Fonction: Anti-KDM6B/JMJD3 Picoband® Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-KDM6B/JMJD3 Picoband® Antibody (ABIN7599752). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human KDM6B/JMJD3 recombinant protein (Position: R1127-R1643).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Ge, C., Ye, J., Weber, C., Sun, W., Zhang, H., Zhou, Y., Cai, C., Qian, G., Capel, B. The histone demethylase KDM6B regulates temperature-dependent sex determination in a turtle species. Science 360: 645-648, 2018. 2. Sen, G. L., Webster, D. E., Barragan, D. I., Chang, H. Y., Khavari, P. A. Control of differentiation in a self-renewing mammalian tissue by the histone demethylase JMJD3. Genes Dev. 22: 1865-1870, 2008. 3. Stolerman, E. S., Francisco, E., Stallworth, J. L., Jones, J. R., Monaghan, K. G., Keller-Ramey, J., Person, R., Wentzensen, I. M., McWalter, K., Keren, B., Heron, B., Nava, C., and 19 others. Genetic variants in the KDM6B gene are associated with neurodevelopmental delays and dysmorphic features. Am. J. Med. Genet. 179A: 1276-1286, 2019.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Kdm6b

Antigène: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Autre désignation: KDM6B

Sujet:

Synonyms: Lysine-specific demethylase 6B, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Lysine demethylase 6B, KDM6B, JMJD3, KIAA0346

Tissue Specificity: Expressed in breast cancer cell lines and in normal breast tissue.

Background: Lysine demethylase 6B is a protein that in humans is encoded by the KDM6B gene. It is mapped to 17p13.1. The protein encoded by this gene is a lysine-specific demethylase that specifically demethylates di- or tri-methylated lysine 27 of histone H3 (H3K27me2 or H3K27me3). H3K27 trimethylation is a repressive epigenetic mark controlling chromatin organization and gene silencing. This protein can also demethylate non-histone proteins such as retinoblastoma protein. Through its demethylation actvity this gene influences cellular differentiation and development, tumorigenesis, inflammatory diseases, and neurodegenerative diseases. This protein has two classical nuclear localization signals at its N-terminus. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Poids moléculaire: 177 kDa

ID gène: 23135

UniProt: O15054

Pathways: L'effet Warburg