PAWR anticorps (AA 13-333)

N° du produit ABIN7599938

Cet anticorps Lapin Polyclonal détecte spécifiquement PAWR dans WB, IHC, ELISA, IF, ICC et FACS. Il présente une réactivité envers Souris et Rat.

Aperçu rapide pour PAWR anticorps (AA 13-333) (ABIN7599938)

Antigène: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Reactivité: Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PAWR est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-PAWR anticorps

Épitope: AA 13-333

Fonction: Anti-PAR4/Pawr Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PAR4/Pawr Antibody Picoband® (ABIN7599938). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived mouse PAR4/Pawr recombinant protein (Position: T13-R333).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/mL, -
1. Donninger, H., Hesson, L., Vos, M., Beebe, K., Gordon, L., Sidransky, D., Liu, J. W., Schlegel, T., Payne, S., Hartmann, A., Latif, F., Clark, G. J. The Ras effector RASSF2 controls the PAR-4 tumor suppressor. Molec. Cell. Biol. 30: 2608-2620, 2010. 2. Duan, W., Zhang, Z., Gash, D. M., Mattson, M. P. Participation of prostate apoptosis response-4 in degeneration of dopaminergic neurons in models of Parkinson's disease. Ann. Neurol. 46: 587-597, 1999. 3. Johnstone, R. W., See, R. H., Sells, S. F., Wang, J., Muthukkumar, S., Englert, C., Haber, D. A., Licht, J. D., Sugrue, S. P., Roberts, T., Rangnekar, V. M., Shi, Y. A novel repressor, par-4, modulates transcription and growth suppression functions of the Wilms' tumor suppressor WT1. Molec. Cell. Biol. 16: 6945-6956, 1996.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur PAWR

Antigène: PAWR (PRKC, Apoptosis, WT1, Regulator (PAWR))

Autre désignation: Pawr

Sujet:

Synonyms: Protein ERGIC-53, ER-Golgi intermediate compartment 53 kDa protein, Gp58, Intracellular mannose-specific lectin MR60, Lectin mannose-binding 1, LMAN1, ERGIC53, F5F8D

Tissue Specificity: Ubiquitous.

Background: PRKC, apoptosis, WT1, regulator, also known as PAWR or Prostate apoptosis response-4 (Par-4), is a human gene. This gene encodes a tumor suppressor protein that selectively induces apoptosis in cancer cells through intracellular and extracellular mechanisms. The intracellular mechanism involves the inhibition of pro-survival pathways and the activation of Fas-mediated apoptosis, while the extracellular mechanism involves the binding of a secreted form of this protein to glucose regulated protein 78 (GRP78) on the cell surface, which leads to activation of the extrinsic apoptotic pathway. This gene is located on the unstable human chromosomal 12q21 region and is often deleted or mutated different tumors. The encoded protein also plays an important role in the progression of age-related diseases.

Poids moléculaire: 45 kDa

ID gène: 114774

UniProt: Q925B0