RCC1 anticorps (AA 14-421)

N° du produit ABIN7600023

L’anticorps Souris Monoclonal anti-RCC1 a été validé pour WB. Il convient pour détecter RCC1 dans des échantillons de Humain.

Aperçu rapide pour RCC1 anticorps (AA 14-421) (ABIN7600023)

Antigène: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RCC1 est non-conjugé

Application: Western Blotting (WB)

Clone: 7B5D2

Détail du produit anti-RCC1 anticorps

Épitope: AA 14-421

Fonction: Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RCC1 Antibody Picoband® (monoclonal, 7B5D2) (ABIN7600023). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human RCC1 recombinant protein (Position: A14-S421).

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
1. Bischoff, F. R., Maier, G., Tilz, G., Ponstingl, H. A 47- kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation. Proc. Nat. Acad. Sci. 87: 8617-8621, 1990. 2. Carazo-Salas, R. E., Guarguaglini, G., Gruss, O. J., Segref, A., Karsenti, E., Mattaj, I. W. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature 400: 178-181, 1999. 3. Chen, T., Muratore, T. L., Schaner-Tooley, C. E., Shabanowitz, J., Hunt, D. F., Macara, I. G. N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis. Nature Cell Biol. 9: 596-603, 2007.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur RCC1

Antigène: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Autre désignation: RCC1

Sujet:

Synonyms: Histone-binding protein RBBP4, Chromatin assembly factor 1 subunit C, CAF-1 subunit C, Chromatin assembly factor I p48 subunit, CAF-I 48 kDa subunit, CAF-I p48, Nucleosome-remodeling factor subunit RBAP48, Retinoblastoma-binding protein 4, RBBP-4, Retinoblastoma-binding protein p48, RBBP4, RBAP48

Background: CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

Poids moléculaire: 47 kDa

ID gène: 1104

UniProt: P18754

Pathways: Chromatin Binding, Protein targeting to Nucleus