RCC1 anticorps (AA 14-421)

N° du produit ABIN7600024

Cet anticorps anti-RCC1 est un anticorps Lapin Polyclonal détectant RCC1 dans WB, ELISA, IHC, IF, IHC (fro), ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour RCC1 anticorps (AA 14-421) (ABIN7600024)

Antigène: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp RCC1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Détail du produit anti-RCC1 anticorps

Épitope: AA 14-421

Fonction: Anti-RCC1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-RCC1 Antibody Picoband® (ABIN7600024). Tested in ELISA, Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human RCC1 recombinant protein (Position: A14-S421).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 4 μg/mL, Human
Immunofluorescence, 4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bischoff, F. R., Maier, G., Tilz, G., Ponstingl, H. A 47- kDa human nuclear protein recognized by antikinetochore autoimmune sera is homologous with the protein encoded by RCC1, a gene implicated in onset of chromosome condensation. Proc. Nat. Acad. Sci. 87: 8617-8621, 1990. 2. Carazo-Salas, R. E., Guarguaglini, G., Gruss, O. J., Segref, A., Karsenti, E., Mattaj, I. W. Generation of GTP-bound Ran by RCC1 is required for chromatin-induced mitotic spindle formation. Nature 400: 178-181, 1999. 3. Chen, T., Muratore, T. L., Schaner-Tooley, C. E., Shabanowitz, J., Hunt, D. F., Macara, I. G. N-terminal alpha-methylation of RCC1 is necessary for stable chromatin association and normal mitosis. Nature Cell Biol. 9: 596-603, 2007.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur RCC1

Antigène: RCC1 (Regulator of Chromosome Condensation 1 (RCC1))

Autre désignation: RCC1

Sujet:

Synonyms: Short transient receptor potential channel 5, TrpC5, Transient receptor protein 5, TRP-5, hTRP-5, hTRP5, TRPC5, TRP5

Tissue Specificity: Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole.

Background: CHC1, also named as RCC1, SNHG3-RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against residues near the C terminus of human RCC1. The geneID has updated as 1104 recently.

Poids moléculaire: 45 kDa

ID gène: 1104

UniProt: P18754

Pathways: Chromatin Binding, Protein targeting to Nucleus