MASP1 anticorps (AA 169-580)

N° du produit ABIN7600246

L’anticorps Lapin Polyclonal anti-MASP1 a été validé pour WB, ELISA et FACS. Il convient pour détecter MASP1 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MASP1 anticorps (AA 169-580) (ABIN7600246)

Antigène: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MASP1 est non-conjugé

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Détail du produit anti-MASP1 anticorps

Épitope: AA 169-580

Fonction: Anti-MASP1 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-MASP1 Antibody Picoband® (ABIN7600246). Tested in WB, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MASP1 recombinant protein (Position: R169-E580). Human MASP1 shares 84.5% and 83.3% amino acid (aa) sequence identity with mouse and rat MASP1, respectively.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Dahl, M. R., Thiel, S., Matsushita, M., Fujita, T., Willis, A. C., Christensen, T., Vorup-Jensen, T., Jensenius, J. C. MASP-3 and its association with distinct complexes of the mannan-binding lectin complement activation pathway. Immunity 15: 127-135, 2001. 2. Degn, S. E., Jensen, L., Hansen, A. G., Duman, D., Tekin, M., Jensenius, J. C., Thiel, S. Mannan-binding lectin-associated serine protease (MASP)-1 is crucial for lectin pathway activation in human serum, whereas neither MASP-1 nor MASP-3 is required for alternative pathway function. J. Immun. 189: 3957-3969, 2012. 3. Dobo, J., Harmat, V., Beinrohr, L., Sebestyen, E., Zavodszky, P., Gal, P. MASP-1, a promiscuous complement protease: structure of its catalytic region reveals the basis of its broad specificity. J. Immun. 183: 1207-1214, 2009.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur MASP1

Antigène: MASP1 (Mannan-Binding Lectin Serine Peptidase 1 (MASP1))

Autre désignation: MASP1

Sujet:

Synonyms: MASP1, CRARF, CRARF1, PRSS5, Mannan-binding lectin serine protease 1, EC 3.4.21.-, Complement factor MASP-3, Complement-activating component of Ra-reactive factor, Mannose-binding lectin-associated serine protease 1, MASP-1, Mannose-binding protein-associated serine protease, Ra-reactive factor serine protease p100, RaRF, Serine protease 5 [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain, Mannan-binding lectin serine protease 1 light chain]

Background: This gene encodes a serine protease that functions as a component of the lectin pathway of complement activation. The complement pathway plays an essential role in the innate and adaptive immune response. The encoded protein is synthesized as a zymogen and is activated when it complexes with the pathogen recognition molecules of lectin pathway, the mannose-binding lectin and the ficolins. This protein is not directly involved in complement activation but may play a role as an amplifier of complement activation by cleaving complement C2 or by activating another complement serine protease, MASP-2. The encoded protein is also able to cleave fibrinogen and factor XIII and may may be involved in coagulation. A splice variant of this gene which lacks the serine protease domain functions as an inhibitor of the complement pathway. Alternate splicing results in multiple transcript variants.

Poids moléculaire: 79 kDa

ID gène: 5648

UniProt: P48740

Pathways: Système du Complément