PIGS anticorps (AA 184-504)

N° du produit ABIN7600375

L’anticorps Lapin Polyclonal anti-PIGS a été validé pour ELISA, WB, IF, FACS et ICC. Il convient pour détecter PIGS dans des échantillons de Humain et Rat.

Aperçu rapide pour PIGS anticorps (AA 184-504) (ABIN7600375)

Antigène: PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))

Reactivité: Humain, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PIGS est non-conjugé

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-PIGS anticorps

Épitope: AA 184-504

Fonction: Anti-PIGS Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PIGS Antibody Picoband® (ABIN7600375). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PIGS recombinant protein (Position: R184-D504).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Efthymiou, S., Dutra-Clarke, M., Maroofian, R., Kaiyrzhanov, R., Scala, M., Alvi, J. R., Sultan, T., Christoforou, M., Nguyen, T. T. M., Mankad, K., Vona, B., Rad, A., Striano, P., Salpietro, V., Guillen Sacoto, M. J., Zaki, M. S., Gleeson, J. G., Campeau, P. M., Russell, B. E., Houlden, H. Expanding the phenotype of PIGS-associated early onset epileptic developmental encephalopathy. Epilepsia 62: e35-e41, 2021. 2. Gross, M. B. Personal Communication. Baltimore, Md. 7/21/2020. 3. Nguyen, T. T. M., Murakami, Y., Wigby, K. M., Baratang, N. V., Rousseau, J., St-Denis, A., Rosenfeld, J. A., Laniewski, S. C., Jones, J., Iglesias, A. D., Jones, M. C., Masser-Frye, D., and 7 others. Mutations in PIGS, encoding a GPI transamidase, cause a neurological syndrome ranging from fetal akinesia to epileptic encephalopathy. Am. J. Hum. Genet. 103: 602-611, 2018.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PIGS

Antigène: PIGS (Phosphatidylinositol Glycan Anchor Biosynthesis, Class S (PIGS))

Autre désignation: PIGS

Sujet:

Synonyms: Semaphorin-3E, SEMA3E, KIAA0331, SEMAH

Tissue Specificity: Detected in the sinusoidal epithelium in the red pulp of spleen. Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

Background: GPI transamidase component PIG-S is an enzyme that in humans is encoded by the PIGS gene. This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.

Poids moléculaire: 65 kDa

ID gène: 94005

UniProt: Q96S52

Pathways: Inositol Metabolic Process, SARS-CoV-2 Protein Interactome