PPP1R15B anticorps (AA 211-657)

N° du produit ABIN7600638

Cet anticorps Lapin Polyclonal détecte spécifiquement PPP1R15B dans WB, ELISA, IF, FACS et ICC. Il présente une réactivité envers Humain.

Aperçu rapide pour PPP1R15B anticorps (AA 211-657) (ABIN7600638)

Antigène: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp PPP1R15B est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-PPP1R15B anticorps

Épitope: AA 211-657

Fonction: Anti-PPP1R15B Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-PPP1R15B Antibody Picoband® (ABIN7600638). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PPP1R15B recombinant protein (Position: Q211-D657).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Harding, H. P., Zhang, Y., Scheuner, D., Chen, J.-J., Kaufman, R. J., Ron, D. Ppp1r15 gene knockout reveals an essential role for translation initiation factor 2 alpha (eIF2-alpha) dephosphorylation in mammalian development. Proc. Nat. Acad. Sci. 106: 1832-1837, 2009.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PPP1R15B

Antigène: PPP1R15B (Protein Phosphatase 1, Regulatory Subunit 15B (PPP1R15B))

Autre désignation: PPP1R15B

Sujet:

Synonyms: Protein NDRG3,N-myc downstream-regulated gene 3 protein,NDRG3,

Tissue Specificity: Ubiquitous. Highly expressed in brain. .

Background: PPP1R15B(Protein phosphatase 1, regulatory subunit 15b), also called CREP, promotes dephosphorylation of the transcription initiation factor EIF2-alpha through recruitment of protein phosphatase-1(PP1) catalytic subunits. The PPP1R15B gene is mapped to chromosome 1q32.1 based on an alignment of thePPP1R15B sequence by Hartz(2010). Harding et al.(2009) obtained Ppp1r15b -/- mice at a mendelian ratio. However, Ppp1r15b -/- newborns were half the size of their wildtype littermates, were notably pale, and failed to nurse, and none survived the first day of postnatal life. Ppp1r15b -/- embryos that were also homozygous for an Eif2-alpha mutation that prevented Eif2-alpha phosphorylation were normalized, including elevated birth size and restored red blood cell count, compared with Ppp1r15b -/- embryos with wildtype Eif2-alpha.

Poids moléculaire: 110 kDa

ID gène: 84919

Pathways: ER-Nucleus Signaling