PDE6B anticorps (AA 25-237)

N° du produit ABIN7600892

Cet anticorps anti-PDE6B est un anticorps Souris Monoclonal détectant PDE6B dans WB, IHC, IF, ICC et FACS. Adapté pour Humain, Souris et Rat.

Aperçu rapide pour PDE6B anticorps (AA 25-237) (ABIN7600892)

Antigène: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PDE6B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 8I2D7

Détail du produit anti-PDE6B anticorps

Épitope: AA 25-237

Fonction: Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2D7)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-PDE6 beta/PDE6B Antibody Picoband® (monoclonal, 8I2D7) (ABIN7600892). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human PDE6 beta/PDE6B recombinant protein (Position: K25-Q237).

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x⁶ cells, Human
1. Hmani-Aifa, M., Benzina, Z., Zulfiqar, F., Dhouib, H., Shahzadi, A., Ghorbel, A., Rebai, A., Soderkvist, P., Riazuddin, S., Kimberling, W. J., Ayadi, H. Identification of two new mutations in the GPR98 and the PDE6B genes segregating in a Tunisian family. Europ. J. Hum. Genet. 17: 474-482, 2009. 2. Tsang, S. H., Woodruff, M. L., Jun, L., Mahajan, V., Yamashita, C. K., Pedersen, R., Lin, C.-S., Goff, S. P., Rosenberg, T., Larsen, M., Farber, D. B., Nusinowitz, S. Transgenic mice carrying the H258N mutation in the gene encoding the beta-subunit of phosphodiesterase-6 (PDE6B) provide a model for human congenital stationary night blindness. Hum. Mutat. 28: 243-254, 2007. 3. Zhao, L., Zabel, M. K., Wang, X., Ma, W., Shah, P., Fariss, R. N., Qian, H., Parkhurst, C. N., Gan, W.-B., Wong, W. T. Microglial phagocytosis of living photoreceptors contributes to inherited retinal degeneration. EMBO Molec. Med. 7: 1179-1197, 2015.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur PDE6B

Antigène: PDE6B (phosphodiesterase 6B, CGMP-Specific, Rod, beta (PDE6B))

Autre désignation: PDE6B

Sujet:

Synonyms: Arylsulfatase A, ASA, Cerebroside-sulfatase, Arylsulfatase A component B, Arylsulfatase A component C, ARSA

Background: Photon absorption triggers a signaling cascade in rod photoreceptors that activates cGMP phosphodiesterase (PDE), resulting in the rapid hydrolysis of cGMP, closure of cGMP-gated cation channels, and hyperpolarization of the cell. PDE is a peripheral membrane heterotrimeric enzyme made up of alpha, beta, and gamma subunits. This gene encodes the beta subunit. Mutations in this gene result in retinitis pigmentosa and autosomal dominant congenital stationary night blindness. Multiple transcript variants encoding different isoforms have been found for this gene.

Poids moléculaire: 98 kDa

ID gène: 5158

UniProt: P35913

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction