APOA1 anticorps (AA 25-264)

N° du produit ABIN7600897

L’anticorps Souris Monoclonal anti-APOA1 a été validé pour WB et ELISA. Il convient pour détecter APOA1 dans des échantillons de Humain.

Aperçu rapide pour APOA1 anticorps (AA 25-264) (ABIN7600897)

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp APOA1 est non-conjugé

Application: Western Blotting (WB), ELISA

Clone: 17G5

Détail du produit anti-APOA1 anticorps

Épitope: AA 25-264

Fonction: Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Apolipoprotein A I APOA1 Antibody Picoband® (monoclonal, 17G5) (ABIN7600897). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Affinity-chromatography

Immunogène: E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264). Mouse Apolipoprotein A I shares 64% and 68.6% amino acid (aa) sequence identity with human and rat Apolipoprotein A I, respectively.

Isotype: IgG1

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
ELISA (Cap), 1-5 μg/mL
1. Brewer, H. B., Jr., Fairwell, T., LaRue, A., Ronan, R., Houser, A., Bronzert, T. J. : The amino acid sequence of human apoA-I, an apolipoprotein isolated from high absorbance lipoproteins. Biochem. Biophys. Res. Commun. 80: 623-630, 1978. 2. Ma J, Liao XL, Lou B, Wu MP (2004). "Role of apolipoprotein A-I in protecting against endotoxin toxicity". Acta Biochim. Biophys. Sin. (Shanghai) 36 (6): 419-24. 3. Yui Y, Aoyama T, Morishita H, Takahashi M, Takatsu Y, Kawai C (1988). "Serum prostacyclin stabilizing factor is identical to apolipoprotein A-I (Apo A-I). A novel function of Apo A-I". J. Clin. Invest. 82 (3): 803-7. 4. Zhang, Y., Zanotti, I., Reilly, M. P., Glick, J. M., Rothblat, G. H., Rader, D. J. : Overexpression of apolipoprotein A-I promotes reverse transport of cholesterol from macrophages to feces in vivo. Circulation 108: 661-663, 2003.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur APOA1

Antigène: APOA1 (Apolipoprotein A-I (APOA1))

Autre désignation: APOA1

Sujet:

Synonyms: Apolipoprotein A-I, Apo-AI, ApoA-I, Apolipoprotein A1, Proapolipoprotein A-I, ProapoA-I, Truncated apolipoprotein A-I, Apolipoprotein A-I (1-242), APOA1

Tissue Specificity: Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease.

Background: Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Poids moléculaire: 25 kDa

ID gène: 335

UniProt: P02647

Pathways: Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism