DDX4 anticorps (AA 3-666)

N° du produit ABIN7601203

L’anticorps Lapin Polyclonal anti-DDX4 a été validé pour WB, ELISA, IHC, FACS, IF et ICC. Il convient pour détecter DDX4 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour DDX4 anticorps (AA 3-666) (ABIN7601203)

Antigène: DDX4 (DEAD Box Protein 4 (DDX4))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DDX4 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Détail du produit anti-DDX4 anticorps

Épitope: AA 3-666

Fonction: Anti-DDX4/MVH Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-DDX4/MVH Antibody Picoband® (ABIN7601203). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunofluorescence, 5 μg/mL, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Abe, K., Noce, T. A DEAD-family protein gene, Ddx4, encoding a murine homolog of Drosophila vasa maps to the distal end of mouse chromosome 13. Mammalian Genome 8: 622-623, 1997. 2. Castrillon, D. H., Quade, B. J., Wang, T. Y., Quigley, C., Crum, C. P. The human VASA gene is specifically expressed in the germ cell lineage. Proc. Nat. Acad. Sci. 97: 9585-9590, 2000. 3. Chu, D. S., Liu, H., Nix, P., Wu, T. F., Ralston, E. J., Yates, J. R., III, Meyer, B. J. Sperm chromatin proteomics identifies evolutionarily conserved fertility factors. Nature 443: 101-105, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur DDX4

Antigène: DDX4 (DEAD Box Protein 4 (DDX4))

Autre désignation: DDX4

Sujet:

Synonyms: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Vasa homolog, DDX4, VASA

Tissue Specificity: Expressed only in ovary and testis. Expressed in migratory primordial germ cells in the region of the gonadal ridge in both sexes.

Background: DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

Poids moléculaire: 79 kDa

ID gène: 54514