MAG anticorps (AA 34-605)

N° du produit ABIN7601399

Cet anticorps anti-MAG Monoclonal Souris (Clone 2G11) (ABIN7601399) détecte spécifiquement MAG dans WB, IHC, FACS et IF. L’anticorps est réactif avec des échantillons de Humain, Souris et Rat.

Aperçu rapide pour MAG anticorps (AA 34-605) (ABIN7601399)

Antigène: MAG (Myelin Associated Glycoprotein (MAG))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp MAG est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 2G11

Détail du produit anti-MAG anticorps

Épitope: AA 34-605

Fonction: Anti-MAG Antibody (monoclonal, 2G11)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-MAG.Antibody (monoclonal, 2G11). Tested in Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human MAG recombinant protein (Position: E34-R605).

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse,Rat Immunofluorescence, 5 μg/mL, Rat Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur MAG

Antigène: MAG (Myelin Associated Glycoprotein (MAG))

Autre désignation: MAG

Sujet:

Background: MAG (Myelin-associated glycoprotein),also known as SIGLEC4A, is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR. It is though to be involved in the process of myelination. MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor.The MAG gene is mapped on 19q13.12. Cleavage of GPI-linked proteins from axons protects growth cones from MAG-induced collapse, and dominant-negative NgR eliminates MAG inhibition of neurite outgrowth. MAG-resistant embryonic neurons were rendered MAG-sensitive by expression of NgR. MAG binds specifically to an NgR-expressing cell line in a GPI-dependent and sialic acid-independent manner. Experiments blocking NgR from interacting with MAG prevented inhibition of neurite outgrowth by MAG. In cultured human embryonic kidney (HEK) cells expressing the NOGO receptor, p75 (NTR) was required for MAG-induced intracellular calcium elevation.

Gene Full Name: myelin associated glycoprotein

Poids moléculaire: 100 kDa

ID gène: 4099

UniProt: P20916

Pathways: Neurotrophin Signaling Pathway