UPF3A anticorps (AA 4-452)

N° du produit ABIN7601625

Cet anticorps Lapin Polyclonal détecte spécifiquement UPF3A dans WB, ELISA, IF, FACS et ICC. Il présente une réactivité envers Humain, Souris et Rat.

Aperçu rapide pour UPF3A anticorps (AA 4-452) (ABIN7601625)

Antigène: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp UPF3A est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Détail du produit anti-UPF3A anticorps

Épitope: AA 4-452

Fonction: Anti-UPF3A Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-UPF3A Antibody Picoband® (ABIN7601625). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human UPF3A recombinant protein (Position: E4-E452).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Chan, W.-K., Bhalla, A. D., Le Hir, H., Nguyen, L. S., Huang, L., Gecz, J., Wilkinson, M. F. A UPF3-mediated regulatory switch that maintains RNA surveillance. Nature Struct. Molec. Biol. 16: 747-753, 2009. 2. Gross, M. B. Personal Communication. Baltimore, Md. 2/28/2011. 3. Kim, V. N., Kataoka, N., Dreyfuss, G. Role of the nonsense-mediated decay factor hUpf3 in the splicing-dependent exon-exon junction complex. Science 293: 1832-1836, 2001.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur UPF3A

Antigène: UPF3A (UPF3 Regulator of Nonsense Transcripts Homolog A (UPF3A))

Autre désignation: UPF3A

Sujet:

Synonyms: Disintegrin and metalloproteinase domain-containing protein 2,ADAM 2,Cancer/testis antigen 15,CT15,Fertilin subunit beta,PH-30,PH30,PH30-beta,ADAM2,FTNB,

Tissue Specificity: Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.

Background: Regulator of nonsense transcripts 3A is a protein that in humans is encoded by the UPF3A gene. This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome 13. Several splice variants encoding different isoforms have been found for this gene.

Poids moléculaire: 51 kDa

ID gène: 65110

UniProt: Q9H1J1