DBT anticorps (AA 46-462)

N° du produit ABIN7601800

L’anticorps anti-DBT Polyclonal Lapin est utilisé pour la détection de DBT dans des échantillons de Humain, Souris et Rat. Il a été validé pour WB, ELISA, IF, ICC, FACS et IP.

Aperçu rapide pour DBT anticorps (AA 46-462) (ABIN7601800)

Antigène: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Reactivité: Humain, Souris, Rat

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp DBT est non-conjugé

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Détail du produit anti-DBT anticorps

Épitope: AA 46-462

Fonction: Anti-DBT Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins

Attributs du produit: Anti-DBT Antibody (ABIN7601800). Tested in WB, ICC/IF, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human DBT recombinant protein (Position: K46-R462). Human DBT shares 89.7% amino acid (aa) sequence identity with mouse DBT.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Immunoprecipitation, 2-4 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Détails sur DBT

Antigène: DBT (Dihydrolipoamide Branched Chain Transacylase E2 (DBT))

Autre désignation: DBT

Sujet:

Synonyms: DBT, BCATE2, Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial, EC 2.3.1.168, 52 kDa mitochondrial autoantigen of primary biliary cirrhosis, Branched chain 2-oxo-acid dehydrogenase complex component E2, BCOADC-E2, Branched-chain alpha-keto acid dehydrogenase complex component E2, BCKAD-E2, BCKADE2, Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, Dihydrolipoamide branched chain transacylase, Dihydrolipoyllysine-residue, 2-methylpropanoyltransferase

Background: The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.

Poids moléculaire: 53 kDa

ID gène: 1629

UniProt: P11182