IRF3 anticorps (AA 5-422)

N° du produit ABIN7601885

Cet anticorps anti-IRF3 est un anticorps Lapin Polyclonal détectant IRF3 dans WB, ELISA, IHC et FACS. Adapté pour Humain.

Aperçu rapide pour IRF3 anticorps (AA 5-422) (ABIN7601885)

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp IRF3 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-IRF3 anticorps

Épitope: AA 5-422

Fonction: Anti-IRF3 Antibody Picoband®

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-IRF3 Antibody (ABIN7601885). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human IRF3 recombinant protein (Position: K5-Q422).

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bellingham, J., Gregory-Evans, K., Gregory-Evans, C. Y. Mapping of human interferon regulatory factor 3 (IRF3) to chromosome 19q13.3-13.4 by an intragenic polymorphic marker. Ann. Hum. Genet. 62: 231-234, 1998. 2. Doyle, S. E., Vaidya, S. A., O'Connell, R., Dadgostar, H., Dempsey, P. W., Wu, T.-T., Rao, G., Sun, R., Haberland, M. E., Modlin, R. L., Cheng, G.IRF3 mediates a TLR3/TLR4-specific antiviral gene program. Immunity 17: 251-263, 2002. 3. Stetson, D. B., Medzhitov, R. Recognition of cytosolic DNA activates an IRF3-dependent innate immune response. Immunity 24: 93-103, 2006.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur IRF3

Antigène: IRF3 (Interferon Regulatory Factor 3 (IRF3))

Autre désignation: IRF3

Sujet:

Synonyms: Interleukin-17C, Il-17c, Cytokine CX2, Il17c

Tissue Specificity: Expressed by epithelial cells after bacterial challenge. Low expression, if any, in lymphocytes.

Background: IRF3 (interferon regulatory factor 3) is a member of the interferon regulatory transcription factor (IRF) family. The IRF3 gene is mapped on 19q13.33. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. IRF3 plays an important role in the innate immune system's response to viral infection. Aggregated MAVS have been found to activate IRF3 dimerization. Although IRF3 increased transcriptional activity from an ISRE-containing promoter, expression of IRF3 as a Gal4 fusion protein did not activate expression of a chloramphenicol acetyltransferase (CAT) reporter gene containing repeats of the Gal4-binding sites. Translocation of IRF3 was accompanied by an increase in serine and threonine phosphorylation. The transcriptional activators CREBBP and EP300 coimmunoprecipitated with IRF3 only subsequent to viral infection, and the authors stated that these are also subunits of DRAF1.

Poids moléculaire: 55 kDa

ID gène: 3661

UniProt: Q14653

Pathways: Signalisation TLR, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades