HSF1 anticorps (C-Term)

N° du produit ABIN7602894

Cet anticorps Lapin Polyclonal détecte spécifiquement HSF1 dans WB, IHC et FACS. Il présente une réactivité avec des échantillons de Humain.

Aperçu rapide pour HSF1 anticorps (C-Term) (ABIN7602894)

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp HSF1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Détail du produit anti-HSF1 anticorps

Épitope: C-Term

Fonction: Anti-HSF1 Antibody

Réactivité croisée (Details): No cross-reactivity with other proteins.

Homologie: identical to the related mouse,rat sequences.

Attributs du produit: Anti-HSF1 Antibody. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the C-terminus of human HSF1, identical to the related mouse and rat sequences.

Isotype: IgG

Information d'application

Indications d'application: Western blot, 0.1-0.25 μg/mL/mL, Human Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human Flow Cytometry (Fixed), 1-3 μg/mL/1x10^6 cells, Human

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Stock: 4 °C,-20 °C

Stockage commentaire: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Date de péremption: 12 months

Détails sur HSF1

Antigène: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Autre désignation: HSF1

Sujet:

Background: HSF1(HEAT-SHOCK TRANSCRIPTION FACTOR 1), also called HEAT-SHOCK FACTOR 1, is a protein that in humans is encoded by the HSF1 gene. The product of HSF1 gene is a heat-shock transcription factor. The International Radiation Hybrid Mapping Consortium maps the HSF1 gene to chromosome 8. HSF1 exists as an inactive monomer in a complex with Hsp40/Hsp70 and Hsp90. Upon stress, such as elevated temperature, HSF1 is released from the chaperone complex and trimerizes. HSF1 is then transported into the nucleus where it is hyperphosphorylated and binds to DNA containing heat shock elements. HSF1's target genes include major inducible heat shock proteins such as Hsp72, and interestingly, noncoding RNA within Satellite III repeat regions. In a novel in vitro system human HSF1 can be activated by nonnative protein, heat, and geldanamycin. HSR1 is constitutively expressed in human and rodent cells and its homologs are functionally interchangeable. Furthermore, Hsf1-deficient mice had a longer free-running period than wildtype littermates.

Gene Full Name: heat shock transcription factor 1

Poids moléculaire: 80 kDa

ID gène: 3297

UniProt: Q00613