FH anticorps (N-Term)

N° du produit ABIN7603168

L’anticorps Souris Monoclonal anti-FH a été validé pour WB et IHC. Il convient pour détecter FH dans des échantillons de Humain, Souris, Rat et Singe.

Aperçu rapide pour FH anticorps (N-Term) (ABIN7603168)

Antigène: FH (Fumarate Hydratase (FH))

Reactivité: Humain, Souris, Rat, Singe

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp FH est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: 9D8

Détail du produit anti-FH anticorps

Épitope: N-Term

Fonction: Anti-FH Antibody Picoband® (monoclonal, 9D8)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-FH Antibody Picoband® (monoclonal, 9D8) (ABIN7603168). Tested in IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: A synthetic peptide corresponding to a sequence at the N-terminus of human FH, which shares 100% and 97.8% amino acid (aa) sequence identity with mouse and rat FH, respectively.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
1. Begley TP, McMurry J (2005). The organic chemistry of biological pathways. Roberts and Co. Publishers. 2. Weaver T (October 2005). "Structure of free fumarase C from Escherichia coli". ActaCrystallogr. D. 61 (Pt 10): 1395-401.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur FH

Antigène: FH (Fumarate Hydratase (FH))

Autre désignation: FH

Sujet:

Synonyms: Fumarate hydratase, mitochondrial, Fumarase, FH,

Tissue Specificity: Expressed in red blood cells, underexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology.

Background: Fumarase (or fumaratehydratase) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular localization in the cytosolic form is established by the absence of the signal sequence found in the mitochondrial variety. This enzyme participates in 2 metabolic pathways: citric acid cycle, reductive citric acid cycle (CO2 fixation), and is also important in renal cell carcinoma. Mutations in this gene have been associated with the development of leiomyomas in the skin and uterus in combination with renal cell carcinoma.

Poids moléculaire: 48 kDa

ID gène: 2271

UniProt: P07954