MLH1 anticorps (AA 501-600) (Cy5)

N° du produit ABIN728837

L’anticorps Lapin Polyclonal anti-MLH1 a été validé pour IF (p) et IF (cc). Il convient pour détecter MLH1 dans des échantillons de Humain.

Aperçu rapide pour MLH1 anticorps (AA 501-600) (Cy5) (ABIN728837)

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Reactivité: Humain

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp MLH1 est conjugé à/à la Cy5

Application: Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc))

Détail du produit anti-MLH1 anticorps (Cy5)

Épitope: AA 501-600

Réactivité croisée: Humain

Homologie: Mouse,Rat,Dog,Cow

Purification: Purified by Protein A.

Immunogène: KLH conjugated synthetic peptide derived from human MLH1

Isotype: IgG

Information d'application

Indications d'application: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Agent conservateur: ProClin

Précaution d'utilisation: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Date de péremption: 12 months

Détails sur MLH1

Antigène: MLH1 (MutL Homolog 1 (MLH1))

Autre désignation: MLH1

Sujet:

Synonyms: FCC2, COCA2, HNPCC, hMLH1, HNPCC2, DNA mismatch repair protein Mlh1, MutL protein homolog 1, MLH1

Background: Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.

ID gène: 4292

UniProt: P40692

Pathways: Réparation de l'ADN, Production of Molecular Mediator of Immune Response